星点设计-效应面法优选冠心康胶囊挥发油提取工艺

Optimization of Extraction Process of Volatile Oil from Guanxinkang Capsule by Central Composite Design-Response Surface Methodology

目的:优选冠心康胶囊中川芎、当归和桂枝挥发油的提取工艺条件。方法:采用水蒸气蒸馏法提取川芎、当归及桂枝中挥发油,以挥发油收率为评价指标,采用星点设计考察加水量、浸泡时间及提取时间对提取工艺的影响,对试验数据进行多元线性回归和二项式拟合,效应面法优选工艺条件,并进行预测分析。结果:由复相关系数可知,指标二项式拟合方程优于多元线性回归方程,确定最佳工艺条件为加9倍量水浸泡80 min,蒸馏提取7.5 h,提取量预测值与理论值偏差<3%。结论:该优选工艺简便、预测性良好,为冠心康胶囊的工业生产提供试验依据。

Objective：To optimize extraction technology conditions of volatile oil from Ligusticum chuanxiong,Angelica sinensis and Cinnamomum cassia in Guanxinkang capsule.Method：Volatile oil from L.chuanxiong,A.sinensis and C.cassia were extracted by steam distillation method,with yield of volatile oil as evaluation index,the amount of water,soaking time and extraction time were investigated by central composite design,test data was fitted by multi-linear equation and second-order polynomial equation,extraction technology conditions were optimized by response surface method,and to predictive analysis.Result：Multiple correlation coefficients from second-order polynomial equation were prior to those from multi-linear equation,optimum extraction process conditions were as follows：soaked 80 min with 9 times the amount of water,distillation time 7.5 h,deviation between observed and predicted values was less than 3%.Conclusion：This optimized process was simple with high predictability,it could provide test basis for industrial production of Guanxinkang capsule.