零陵香水溶性多糖组分的GC-MS分析

Analysis of Water-soluble Polysaccharide Components from Herba Lysimachiae Foenum-graeti by GC-MS

目的:鉴定零陵香水溶性多糖组分,为建立专属性的质量评价指标和中药药效组分新药研发奠定基础。方法:采用水提醇沉法制备零陵香粗多糖,Sevag法除蛋白、H2O2脱色、透析得到精制多糖,衍生化后用GC-MS分析。结果:零陵香水溶性多糖主要组分依次为鼠李糖醇-阿拉伯糖-核糖-葡萄糖-木糖,其相对含量比例为1.03∶9.17∶4.74∶18.95∶17.11。结论:GC-MS联用法可快速鉴定零陵香水溶性多糖组分。

Objective：Identifying the water-soluble polysaccharide components of Herba Lysimachiae Foenum-graeti,to establish the quality of specificity evaluation index and Chinese medicine＇s active components alignment,which laid the foundation of new drug development.Method：The preparation of polysaccharides was extracted by the methods of water extracting-alcohol precipitating,then Sevag method sinking in protein,H2O2 bleaching and the use of dialysis method refined.The derivative change of the refined polysaccharide was analyzes by GC-MS.Result： The polysaccharide of Herba Lysimachiae Foenum-graeti is composed of rhamnitol,arabinose,ribose,glucose,and xylose,the proportion of it is 1.03∶9.17∶4.74∶18.95∶17.11.Conclusion：GC-MS could rapidly ascertain the water-soluble polysaccharide components of Herba Lysimachiae Foenum-graeti.The water-soluble polysaccharide of Herba Lysimachiae Foenum-graeti can be used as a component of the quality evaluation index and drug components of the new drug research based material.