摘要
目的:鉴定零陵香水溶性多糖组分,为建立专属性的质量评价指标和中药药效组分新药研发奠定基础。方法:采用水提醇沉法制备零陵香粗多糖,Sevag法除蛋白、H2O2脱色、透析得到精制多糖,衍生化后用GC-MS分析。结果:零陵香水溶性多糖主要组分依次为鼠李糖醇-阿拉伯糖-核糖-葡萄糖-木糖,其相对含量比例为1.03∶9.17∶4.74∶18.95∶17.11。结论:GC-MS联用法可快速鉴定零陵香水溶性多糖组分。
Objective:Identifying the water-soluble polysaccharide components of Herba Lysimachiae Foenum-graeti,to establish the quality of specificity evaluation index and Chinese medicine's active components alignment,which laid the foundation of new drug development.Method:The preparation of polysaccharides was extracted by the methods of water extracting-alcohol precipitating,then Sevag method sinking in protein,H2O2 bleaching and the use of dialysis method refined.The derivative change of the refined polysaccharide was analyzes by GC-MS.Result: The polysaccharide of Herba Lysimachiae Foenum-graeti is composed of rhamnitol,arabinose,ribose,glucose,and xylose,the proportion of it is 1.03∶9.17∶4.74∶18.95∶17.11.Conclusion:GC-MS could rapidly ascertain the water-soluble polysaccharide components of Herba Lysimachiae Foenum-graeti.The water-soluble polysaccharide of Herba Lysimachiae Foenum-graeti can be used as a component of the quality evaluation index and drug components of the new drug research based material.
出处
《中国实验方剂学杂志》
CAS
北大核心
2012年第19期129-131,共3页
Chinese Journal of Experimental Traditional Medical Formulae
关键词
零陵香
水溶性多糖
药效组分
GC-MS
质量评价
Herba Lysimachiae Foenum-graeti
water-soluble polysaccharide
active components alignment
GC-MS
quality evaluation