地锦草乙醇提取物对白色念珠菌性阴道炎大鼠模型的治疗作用

Therapeutic Effects of Ethanol Extracts from Euphorbia humifusa on Monilial Vaginitis in Rats

目的:观察地锦草乙醇提取物对大鼠白色念珠菌性阴道炎模型的治疗效果及其相关机制。方法:在卵巢切除加乙烯雌酚联合氢化可的松注射的基础上予白色念珠菌感染法制备大鼠白色念珠菌性阴道炎模型。并给予地锦草乙醇提取物治疗,连续7d。治疗结束后,取阴道分泌物进行真菌培养;行阴道灌洗,ELISA法测定灌洗液中单核细胞趋化蛋白-1(MCP-1)、巨噬细胞炎性蛋白-2(MIP-2)水平;切取阴道组织行组织病理学观察。结果:与正常组比较,模型组大鼠阴道分泌物培养出的白色念珠菌落数显著增多(P<0.001),地锦草乙醇提取物治疗组大鼠阴道分泌物培养出的白色念珠菌落数显著低于模型组(P<0.01);ELISA检测显示模型组大鼠阴道灌洗液中MCP-1及MIP-2水平显著升高;(P<0.001);地锦草提乙醇取物治疗组大鼠阴道灌洗液中MCP-1及MIP-2水平显著低于模型组(P<0.01);阴道组织病变在光镜下观察发现,实验动物造模成功后,阴道充血、分泌物增多。给地锦草乙醇提取物治疗7d后,阴道局部充血病变显著减轻,分泌物显著减少。结论:地锦草可以通过抑制真菌的生长,减少有关趋化因子的产生,从而发挥了对霉菌性阴道炎的治疗作用。

Objective：To study the effect of ethanol extracts from Euphorbia humifusa on monilial vaginitis in rats.Method： After the candidal vaginitis model establishment,ethanol extracts from E.humifusa was intragastrically administered daily for 7 days.Candida albicans of the vaginal exudates were cultured and analyzed.The levels of MCP-1and MIP-2 in vaginal lavage fluid were detected by enzyme-linked immunosorbent assay（ELISA）method.The pathological changes of vaginal tissue were examined under light microscope.Result： E.hmuifusa ethanol extracts inhibited the C.albicans significantly（P0.001） and decreased the MCP-1and MIP-2 levels in vaginal lavage fluid remarkably.In addition,the ethanol extracts ameliorated the vaginal pathological changes significantly（P0.001）.Conclusion： E.humifusa ethanol extracts has remarkable antifungal activity by inhibiting the C.albicans.