六月青多糖对四氯化碳诱导的大鼠肝纤维化的影响

Effect of Liuyueqing Polysaccharide on Liver Fibrosis Induced by Carbon Tetrachloride in Rats

目的:研究六月青多糖(LYQP)对四氯化碳(carbon tetrachloride,CCl4)所致大鼠肝纤维化的干预作用及机制。方法:SD大鼠随机分成两组,模型组大鼠采用50%CCl4花生油溶液1 mL.kg-1ig造模,每周2次,连续7周,正常组用生理盐水代替。将病理检查确认肝纤维化已形成的SD大鼠60只随机分为LYQP(600,300,150 mg.kg-1)组,秋水仙碱(0.2 mg.kg-1)组,CCl4模型对照组,每组12只。于造模的第8周开始给药。正常组和CCl4模型对照组ig生理盐水,药物组ig LYQP和秋水仙碱,每日1次,连续ig给药4周。检测大鼠血清丙氨酸转氨酶(ALT)、天冬氨酸转氨酶(AST)、谷胱甘肽-过氧化物酶(GSH-Px)活性以及肝组织中羟脯氨酸(Hyp)的含量,采用Masson染色观察肝组织纤维化程度。结果:LYQP(600,300,150 mg.kg-1)组ALT和AST活性为(13.53±2.30),(19.89±2.13),(26.31±2.87)U.mL-1,(17.14±2.96),(24.85±3.14),(33.63±3.91)U.mL-1,与模型对照组比较,LYQP可明显降低CCl4致大鼠纤维化血清ALT和AST活性;GSH-Px活性为(710.4±35.98),(653.8±25.36),(612.0±27.96)U.mg-1,Hyp含量为(0.382±0.017),(0.446±0.022),(0.517±0.024)mg.g-1与模型对照组比较,LYQP可明显增高GSH-Px活性和降低Hyp含量;Masson染色显示各药物组干预的大鼠肝脏胶原纤维均有所减少。结论:六月青多糖可减轻CCl4诱导的肝纤维化。

Objective：To study the effect of the Liuyueqing polysaccharide against CCl4-induced liver fibrosis in rats and its mechanism.Method：SD rats were divided into two groups randomly： hepatic fibrosis model group and normal group.The model group of rats with hepatic fibrosis was established by 1 mL · kg-1 of 50% CCl4,ig,twice per week for continuous 7 weeks.The rats in the normal control group were given normal saline（NS）.Sixty SD rats with hepatic fibrosis confirmed by the pathological inspection was randomly divided into LYQP（600,300,150 mg · kg-1） groups,colchicine（0.2 mg · kg-1） group and model group.All rats were treated with drugs or NS for four consecutive weeks by ig,once a day.The activity of alanine aminotransferase（ALT）,aspartate aminotransferase（AST）,and glutathione synthesis（GSH-Px） in serum of rats and the content of hydroxyproline（Hyp） in hepatic tissue were determined.The extent of liver fibrosis was observed by Masson staining.Result：LYQP significantly decreased the activity of ALT and AST in rats with liver fibrosis.Compared with model control group,the content of Hyp in hepatic tissue was decreased significantly.The activity of GSH-Px was significantly increased.Masson staining showed that the collagen fibers in every treated group were less than model control group.Conclusion：Liuyueqing polysaccharide can relieve liver fibrosis induced by CCl4.