丹参-山楂协同抗大鼠动脉粥样硬化的实验研究

Cooperative Effect of Salvia Miltiorrhiza and Hawthorn for Resisting Atherosclerosis in Rats

目的:观察丹参-山楂提取物(水浸膏)单用及合用对大鼠动脉粥样硬化模型的干预,探讨两药间的交互协同作用。方法:雄性Wistar大鼠随机分为空白对照组和造模组,造模组采用ip维生素D3叠加卵清白蛋白激发免疫反应的方法,在高脂饮食的基础上诱导大鼠动脉粥样硬化(AS)。造模8周确定造模成功后,将造模动物分为模型组,丹参组,山楂组,丹参-山楂合用组。各组经口给予相应的药物,连续4周。给药结束后,检测各组大鼠血脂水平和丙二醛(MDA)、超氧化物歧化酶(SOD),NO水平。苏木素伊红(HE)染色观察主动脉组织形态学变化。结果:各给药组均可降低TC,TG,低密度脂蛋白胆固醇(LDL-C),MDA水平,提高高密度脂蛋白胆固醇(HDL-C),SOD,NO水平,合用组效果更明显(P<0.05)。结论:山楂丹参具有协同作用,二者联用效果更明显。

Objective：To investigate anti-atherosclerosis effect in rats of the extracts of Radix Salviae Miltiorrhizae（RSM）,Fructus Crataegi（FC）,as well as their interaction.Method：Male Wistar rats were randomly divided into 2 groups： normal group and model group.The atherosclerotic model was established by injection of VD3 and ovalbumin,while fed with high cholesterol diet.After the model was determined successfully,all model rats were divided into normal group,model group,RSM group,FC group,mixture of RSM and FC group.Each group was given the corresponding drug for 4 weeks.After the end of treatment,blood serum were analyzed for blood-fat levels,the contents of MDA,SOD and NO,observing ortic organization morphological changes by the method of（HE） dyeing.Result： The TC,TG,LDL-C and MDA level were reduced,while the HDL-C,the serum SOD,NO were raised in the drug intervention groups.And for many indexes,there was obvious（P0.05） effect for the mixture of RSM and FC group.Conclusion：RSM and FC show synergistic effect,the combined effect is better.