补肾益髓对辐射损伤小鼠造血功能的影响——对辐射小鼠外周血相影响的研究

Influences of Tonifying the Kidney to Benefit Marrow on Peripheral Blood in Irradiated Mice

目的:探讨补肾益髓方药(益髓生血颗粒)对辐射损伤致小鼠肾虚髓损模型的治疗作用。方法:采用60Coγ射线3.5 Gy全身一次性照射,造成小鼠骨髓造血抑制、外周血象有形成分减少,致小鼠肾虚髓损。用益髓生血颗粒高、中、低剂量(含生药23.6,11.8,5.9 g.kg-1,ig)照射前预防给药2 d,造模后再连续给药14 d。在照射前、照射后1,3,5,7,11,13,15,17,21 d检测外周血象。结果:与模型组比较,阳性药粒细胞集落刺激因子(G-CSF)在造模后1～17 d白细胞数显著升高(P<0.05,P<0.01);益髓生血颗粒各剂量组均能升高白细胞,高、中、低剂量组分别在造模后1,5,7,13,17 d,促进白细胞数升高(P<0.05或P<0.01);照射后各组的红细胞、血红蛋白均有一定程度的下降,G-CSF和益髓生血颗粒各剂量组红细胞数在15,17 d高于模型组(P<0.05或P<0.01);模型组血小板计数在照后5 d开始下降,照后7 d达最低值,此后缓慢回升,而用药组动物从照射后5 d起较模型组恢复快,其中高剂量组在5,11,13 d、中剂量在13 d明显高于模型组(P<0.05,P<0.01,P<0.01)。结论:益髓生血颗粒各剂量组均能显著升高辐射损伤致小鼠外周血白细胞数,能促进辐射损伤小鼠外周血红细胞、血红蛋白的恢复,促进照射小鼠外周血血小板数的回升,补肾益髓方药具有补肾填精保护骨髓促进造血作用。

Objective：To explore the influence of Yisui Shengxue granule（YSG） on the kidney medullary loss in mice model induced by radiation damage.Method：Whole body irradiation of 60Coγ-ray was used to induce mice model of kidney medullary loss.The model showed bone marrow hematopoietic inhibition and reduction in visible components in peripheral blood.YSG（23.6,11.8,5.9 g · kg-1,ig） was preventively given 2 days before irradiation and successively given 14 days after irradiation.Peripheral blood indexes were examined before radiation and at the 1,2,5,7,9,11,13,15,17,19 and 21 days after radiation.Result：In the positive drug（G-CSF） group,the WBC count was significantly increased after radiation at the 1-17 days compared with those in model group（P0.05,P0.01）.The WBC count in three dosage groups of YSG at the 1,5,7,13 and 17 days after radiation was elevated（P0.05 or P0.01）.The RBC count and hemoglobin were descended to a certain extent after irradiation.The RBC count in the group of positive drug（G-CSF） and all dose groups of YSG was higher than that in the model group（P0.05 or P0.01）.Platelet count was decreased in the 5 day after radiation and reached the lowest level in the 7 day and then rose slowly in model group.However,the platelet count in the treatment group was rapidly recoveried from the 5 day after radiation,and those in the high dosage group were much higher than those in the model group in 5,11 and 13 days,and that in the medium dosage group in the 13 day was higher than that in the models（P0.05,P0.01,P0.001）.Conclusion：All dose groups of YSG can increase the WBC count significantly after radiation damage and promote the RBC,hemoglobin and platelet count recovery.The results suggest that the recipes of promoting kidney and tonifying bone have the function of supplying the kidney essence and protecting marrow and also can promote hematopoietic.