均匀设计法筛选三七、山楂有效部位组合物剂量配比

Uniform Design for Optimizing Proportion of Effective Components from Panax Notoginseng and Crataegus pinnatifida Bge

目的:研究确定三七、山楂(SS)组合物中2个有效部位最佳配比剂量的有效方法。方法:按照U6(64)表将小鼠随机分为山楂叶总黄酮∶三七叶总皂苷200∶15,100∶1.875,50∶30,25∶3.75,12.5∶60,6.25∶7.5(mg.kg-1)6个剂量给药组,连续给药5 d后,采用小鼠腹腔注射异丙肾上腺素和断头致急性脑缺血两种模型观察小鼠存活时间,分别得出多元回归方程,推算2个有效部位的最佳配比。结果:确定SS组合物中山楂叶总黄酮∶三七叶总皂苷最佳组合比例为20∶15 mg.kg-1,即配比为4∶3,此时药效作用明显(P<0.05)。结论:运用均匀设计与药效学验证相结合的方法可确定有效部位组合物的最佳配比。

Objective：To explore effective way to optimize the proportion of effective components from Panax notoginseng and Crataegus pinnatifida Bge.Method：According to table U6（64） in uniform design,mice were divided into 6 treatment groups,the ratios between flavonoids of Crataeguspinnatifida and total saponins of P.notoginseng were 200∶15,100∶1.875,50∶30,25∶3.75,12.5∶60,6.25∶7.5（mg · kg-1）accordingly.The treatments lasted for 5 days.Then,survival time of mice were evaluated by two models,isoproterenol injection intraperitoneally and acute cerebral ischemia induced by broken neck.The best proportion of effective components was calculated by multiple regression equations.Result：The best ratio appeared to be 20∶15 mg · kg-1,namely,the ratio of 4∶3.The proportion could prolong the survival time of mice with acute cerebral ischemia（P0.05）.Conclusion：The combination of uniform design and animal models in pharmacodynamics is an effective way to optimize the proportion of Chinese medicinal components.