前列爽颗粒对慢性前列腺炎模型大鼠血清中IL-10,TNF-α含量的影响

Effects of Qian Lie Shuang Granule on the Expression of IL-10, TNF-α in the Rat Model for Chronic Abacterial Prostatitis

目的:观察前列爽颗粒对慢性前列腺炎(CAP)模型大鼠血清,白细胞介素-10(IL-10)和肿瘤坏死因子-α(TNF-α)表达的影响,并初步探讨其作用机制。方法:将60只SD大鼠随机分为正常对照组、CAP模型组、前列回春胶囊组(0.18 g.kg-1)、前列爽低剂量组(0.79 g.kg-1)、前列爽中剂量组(1.58 g.kg-1)、前列爽高剂量组(3.15 g.kg-1),每组10只。观察大鼠前列腺病理形态学改变,ELISA法检测IL-10,TNF-α。结果:CAP模型大鼠的组织病理学改变符合CAP的特点,治疗组炎症明显消退。模型组血清中IL-10,TNF-α水平明显高于正常组(P<0.05),前列回春胶囊组、前列爽颗粒中、高剂量组血清TNF-α水平明显低于模型组(P<0.05),前列回春胶囊组、前列爽颗粒中、高剂量组,血清中IL-10水平明显升高。结论:前列爽颗粒可通过调节抗炎/促炎因子分泌而发挥治疗CAP的作用。

Objective： Investigate the influence of Qianlie Shuang granule on intereukin-10（IL-10）,tumor necrosis factor-α（TNF-α） in serum of chronic abacterial prostafitis（CAP） rats.Method： Sixty aged sprague dawley（SD） rats were randomly divided into six groups： control group,model group,Qianlie Huichun granule group,Qianlie Shuang low dose group,Qianlie Shuang medium dose group and Qianlie Shuang high dose group.The changes of prostate gland inflammation was investigated.The expression of IL-10 and TNF-α in rat prostatic tissue was detected by immunohistochemistry.Result： The inflammation of model group were similar to those in clinical CAP,the inflammation of treatment groups were obviously degraded.The IL-10 and TNF-α in model group were obviously higher than in control group（P 0.05）.The TNF-α in Qianlie Huichun granule group,Qianlie Shuang medium dose group and high dose group were obviously lower than in model group.The IL10 in those groups were obviously higher than in model group.Conclusions： Qianlie Shuang granule can regulate the secretion of inflammatory cytokines,which can be one of the mechanisms in the therapy of CAP.