治泻方对小肠运动与吸收的实验研究

Experimental investigation of Zhixie recipe on small intestinal motion and absorption

目的通过本实验，观察治泻方对小鼠小肠运动功能及对兔小肠吸收功能的影响，探讨治泻方的药效机理。方法用治泻方药液灌喂小鼠，以蒸馏水灌喂作为对照，然后各组灌喂印度墨汁，处死小鼠后记录墨汁在小肠移动的长度；将治泻方药液与台式液混合，分别配制成浓度为5％泻台液、10％泻台液，并以台式液作为对照液，将这三组药液分别注入兔小肠，然后抽出各段肠液进行氯离子测定。结果在体小肠运动实验中A、B、C三组小鼠墨汁移动的平均百分率分别为52．55％（SD=8．24）、54．35％（SD=12．53）、59．45％（SD=14．17），其中A组、B组分别与c组比较，差异均无统计学意义（P〉0．05）；在小肠吸收功能实验中5％、10％泻台液与台式液比较，差异均有统计学意义（矿值分别为6．92、46．18，P〈0．01），表明5％、10％泻台液均可促进小肠对氯离子的吸收，其中10％泻台液作用更佳。结论治泻方不影响小鼠在体肠管的运动功能并能促进兔小肠吸收功能。

Objective To investigate the effects and mechanism of Zhixie recipe on small intestinal motility of rats and absorption ability of rabbits. Methods Three groups of rats, one was fed with distilled water, the another two were fed with Zhixie recipe liquid of 1 g/ml and 2 g/ml. Then all three groups were fed with ink, and the moving distance of the ink in small intestines was measured to cheek the small intestinal motility; Three mixtures of Zhixie recipe liquid and desktop solution with 0%, 5% and 10% Zhixie recipe liquid were injected into rabbits, the Chloride ion concentration in small intestines was measured to cheek the absorption ability. Results The average moving distance percentage of ink in the small intestines of the three groups of rats was 52.55% （SD = 8.24）, 54.35% （SD = 12.53 ） and 59.45% （SD = 14.17 ） of the total intestine length respectively. It showed that Zhixie recipe had no influence on small intestinal motility （P〉0.05） ; Small intestines absorption experiments revealed that the small intestinal absorption ability was quite different between the rabbits with desktop solution and the rabbits with mixtures of Zhixie recipe liquid and Desktop solution （5% and 10%） , with x^2=6.92 and 46.18, P〈0.01. The mixtures with 5% and 10% Zhixie recipe liquid improved the small intestinal absorption ability, and the effect of the mixture with 10% was even stronger. Conclusion Zhixie recipe showed no influence on small intestinal motility, but it improved the small intestinal absorption ability.