葡萄糖-6-磷酸异构酶抗原检测在类风湿关节炎诊断和病情评估中的作用

目的探讨葡萄糖-6-磷酸异构酶（GPI）抗原在类风湿关节炎（RA）临床诊断和病情评估中的作用。方法采用ELISA法检测116例RA患者、50例非RA患者及80例健康人血清中的GPI抗原表达水平，同时检测类风湿因子（RF）。结果RA患者血清RF和GPI抗原阳性表达率明显高于非RA患者和健康人（P〈0．05），且GPI抗原阳性表达率高于RF阳性表达率；GPI抗原检测的敏感性（78．4％）和特异性（97．7％）均高于RF（67．2％，85．4％），差异有统计学意义（P〈0．05）；活动性RA患者GPI抗原的表达水平明显高于非活动性RA患者（P〈0．05）；不同的x线分期GPI抗原水平差异有统计学意义（P〈0．05）。结论GPI抗原检测对RA的诊断有较高的敏感性和特异性，并与RA的活动性及病程进展相关，可应用于RA的临床诊断和病情评估。

Objective To investigate the role of Glucose-6-phosphate isomerase antigen detection in the clinical diagnosis and condition assessment of rheumatoid arthritis. Methods The expression of serum GPI antigen and rheumatoid factor （RF） in 116 patients with RA, 50 cases ofnon-RA patients, and 80 healthy human were detected by ELISA. Results The positive expression rate of serum RF and GPI antigen in RA patients was significantly higher than that of non- RA patients and normal control group （ P〈0.05 ） , and the positive expression rate of GPI antigen was higher than that of RF. The detection sensitivity of GPI antigen （ 78.4% ） and specificity （ 97.7% ） were higher than that of RF （ 67.2%, 85.4% ） （ P〈0.05 ） .The expression of GPI antigen in active RA patients were significantly higher than that of non-active RA patients （ P〈0.05 ） .There was a significant difference in expression of GPI antigen between the different X-ray stages （ P〈0.05 ） . Conclusion The detection of GPI antigen for the diagnosis of RA had a good sensitivity and specificity. It had a relation with RA activity and disease progression. It can be applied to clinical diagnosis and condition assessment of RA.