应用奇异变形杆菌多克隆抗体提高沙门菌分离鉴定准确性的研究

Increased isolation and identification accuracy for Salmonella by introduction of polyclonal antibody against Proteus mirabilis

在沙门菌检测过程中,奇异变形杆菌经常作为一种假阳性菌而出现。为提高沙门菌分离鉴定的准确性,我们制备了奇异变形杆菌的多克隆抗血清。抗血清经protein G抗体纯化柱纯化,与奇异变形杆菌混合后,在扫描电镜下观察二者的相互作用,分析该抗体是否能够在体外特异性的裂解奇异变形杆菌。经扫描电镜分析,制备的多克隆抗体在2h后将奇异变形杆菌裂解。同时,将纯化的抗体分别添加到氯化镁孔雀绿肉汤(MM)和亚硒酸盐胱氨酸增菌液(SC)中分析对奇异变形杆菌的抑菌效果。在实际应用中,针对临床的100多份样本,进行改进方法与常规方法的比较,结果显示,改进的方法能够有效排除因奇异变形杆菌带来的假阳性干扰,明显提高了沙门菌分离鉴定的准确性。

Proteus mirabilis is one of the false positive bacteria in Salmonella detection.To increase the isolation accuracy for Salmonella,we prepared polyclonal antisera.The antisera which was purified with protein G column was mixed with Proteus mirabilis and then analyzed by scanning electronic microscopy.Proteus mirabilis was destroyed after 2 hours incubation with its antibody in vitro.In addition,purified antibody was added into Rappaport＇s broth（MM） and selenite cysteine broth（SC） to evaluate the microbicidal effect for Proteus mirabilis.Comparison between modified and traditional methods was carried out with more than 100 clinical samples.The results showed that the modified method could eliminate the interference caused by Proteus mirabilis and increase the isolation accuracy of Salmonella.