辣根过氧化物酶催化反应产物的电化学行为

A study on the electrochemical behavior of the product of enzymatic catalytic reaction by horseradish peroxidase

用多种电化学分析方法研究了辣根过氧化物酶催化反应产物 2 ,3-二氨基吩嗪 (DAP)的电化学行为。在不同介质中探讨了测定 DAP的灵敏度 ,并在 p H9.1 5的 Britton- Robinson缓冲溶液中研究了 DAP电化学还原的电极过程。实验表明 ,在 p H9.1 5的 Briton- Robinson缓冲溶液中 ,DAP在滴汞电极上的还原为一不可逆的 。

The Electrochemical behavior of the product, 2,3 diaminophenazine (DAP), of the enzymatic catalytic reaction by horseradish peroxidase was studied by various electrochemical methods.The sensitivity and interference factors for determining DAP were investigated.The reduced process of DAP on dropping mercury electrode was discussed in pH 9 15 Britton Robinson buffer.In the buffer, the electrochemical process was irreversible,non adsorptive and two electron transferring.