外源Ca^(2+)处理对高温胁迫下半夏植株保护效应及主要成分积累规律的影响

Effect of exogenous Ca^(2+) on protective infection of Pinellia ternata and accumulation of major components under high temperature stress

目的:研究外源Ca2+在高温条件下对半夏植株的保护效应及对主要成分积累的影响。方法:采用无土栽培,不同浓度外源Ca2+处理;测定半夏叶片抗逆性指标,统计半夏倒苗率,并测定不同部位草酸、块茎中总生物碱、总有机酸及鸟苷含量。结果:在较低浓度Ca2+处理下,半夏倒苗率高于其他处理。随着Ca2+浓度升高,半夏叶片中SOD,POD活性呈现先升高后下降的趋势,叶片Pro含量则呈现先降后升的趋势;半夏叶片及块茎中可溶态草酸含量均低于未处理可溶态草酸含量,随着Ca2+浓度的升高,半夏叶片及块茎中可溶态草酸含量呈先升后降的趋势;产量的变化趋势与可溶态草酸含量变化一致;当Ca2+质量浓度为400 mg.L-1,半夏块茎总生物碱和鸟苷含量最高,低浓度Ca2+处理下,半夏块茎总游离有机酸含量较高。结论:通过外源Ca2+处理,半夏抗热能力有显著提高,倒苗时间有所推迟,生长期延长,产量有所提高。

Objective： To study the effect of exogenous Ca2＋ on protective infection of Pinellia ternata and accumulation of major components under high temperature stress. Method： The soilless cultivation experiment was applied, stress resistance index of P. ternata leaves, statistics the rate of lodge P. ternata,the content of oxalate in different places in the plant, the content of total alkaloids, total organic acids and glucosine in P. ternata tubers were measured based on different concentrations of exogenous Ca2＋. Result： The test results showed that, at lower concentrations of Ca2＋ treatments, the rate of lodge P. ternata was higher than that of the others. With Ca2＋ concentration increasing, activities of SOD and POD initially increased and then decreased, however, proline level tended to be down then up. Soluble oxalic acid content was lower than the content of unhandled treatment in P. ternata leaves and tubers; with Ca2＋ concentration increasing, soluble oxalic acid content and yield showed a tendency of decrease after increase in the leaves and tubers. Compared with other treatments, spraying 400 mg·L-1 Ca2＋ significantly enhanced the accumulation of total alkaloid and guanosine in P. ternata tubers. At Lower concentrations of Ca2＋, the content of total free organic acid was higher in the tuber. Conclusion： With the treatment of Ca2＋ the capacity of heat resistance was improved in P. ternata plants, the rate of lodge P. ternata was postponed, growing period was extended and corresponding production has increased by spraying exogenous Ca2＋.