少毛北前胡化痰作用的谱效关系研究

Spectrum-effect relationship of reducing phlegm effect of Peucedanum harrysmithii var. subglabrum

目的:分析少毛北前胡氯仿提取部位高效液相色谱法(HPLC)指纹图谱与化痰药效关联性,以确立其化痰"药效成分组"。方法:通过HPLC测定分析少毛北前胡氯仿提取部位HPLC指纹图谱,用小鼠酚红化痰法研究其化痰作用,急支糖浆为阳性对照药(12 mL.kg-1),给小鼠灌胃氯仿提取物6 d(1.4 g.kg-1),用分光光度计测定小鼠气管的酚红分泌量,然后用灰关联度分析方法研究其谱效关系。结果:少毛北前胡氯仿提取部位的化痰作用是其所有化学成分共同作用的结果,各特征峰代表各自化学成分,其对化痰作用贡献的大小顺序(按特征峰编号)为13>12>16>18>19>6>20>14>1>11>15>10>17>2>5>4>7>3>8>9,其中1,3,4,10,13,16号特征峰鉴定为异紫花前胡苷元,补骨脂素,花椒毒素,Pd-Ib,蝉翼素,peuformosin。结论:少毛北前胡氯仿提取部位具有较强化痰活性,其HPLC指纹图谱与化痰作用之间有一定对应关系。

Objective： To analyze the relationship between high-performance liquid chromatography （HPLC） fingerprints of the chloroform extract fractions of Peucedanum harrysmithii var. subglabrum （PHS） and its phlegm-reducing effect, in order to estab- lish ＂active component group for reducing phlegm＂. Method： HPLC was adopted to determine and analyze HPLC fingerprints of chloro- form extract fractions of PHS. Phenol red expectorant experiment was used to observe the phlegm-reducing effect in mice. Mice were ad- ministered intragastrically with chloroform extract fractions for 6 days （ 1.4 g · kg -1 ） , with acute bronchitis syrup as the positive con- trol drug （ 12 mL · kg-1 ）. The phenol red secretion in mice was determined by spectrophotometer. Then the grey relational analysis was used to study the spectrum-effect relationship. Result ： The phlegm-reducing effect of the chloroform extract fractions of PHS were resul- ted from the combined effect of all of its chemical components. Its various characteristic peaks represented different chemical compo- nents, and the order of their contributions to the phlegm-reducing effect was （number of peaks） 13 〉 12 〉 16 〉 18 〉 19 〉 6 〉 20 〉 14 〉 1 〉 11 〉 15 〉 10 〉 17 〉2 〉5 〉4 〉7 〉3 〉8 〉9, in No. 1, 3,4, 10, 13 and 16 characteristic peaks were identified as marmesin, pso- ralen, xanthotoxin, Pd-Ib, pteryxin and peuformosin. Conclusion ： The chloroform extract fractions of PHS show strongly phlegm-reduc- ing effect. There may be certain relationship between their HPLC fingerprint and phlegm-reducing effect.