逍遥方3种制剂的疏肝健脾比较研究

Comparative study on effect of three preparations of Xiaoyao formula on soothing liver and strengthening spleen

目的:比较不同工艺所得逍遥方3种制剂(逍遥胶囊、逍遥丸、逍遥汤剂)的疏肝健脾作用。方法:强迫游泳、限制活动及隔日喂食方法制备肝郁脾虚大鼠模型,大鼠随机分为正常对照组、模型组、西沙必利组(2.7 mg.kg-1)、逍遥汤组(1.62 g.kg-1)、逍遥丸组(1.62 g.kg-1)、逍遥胶囊高、中、低剂量组(3.24,1.62,0.81 g.kg-1),灌胃给药3周,利用高效液相色谱-电化学检测法检测其血清中NE,DA,5-HT含量;放免法测定血浆中生长抑素(SS)、胃动素(MTL)含量;免疫组化法测定组织中生长抑素(SS)、胃动素(MTL)的表达。结果:与模型组相比,逍遥汤组NE,MTL含量显著升高,5-HT和SS含量显著降低(P<0.01);逍遥胶囊1.62 g.kg-1组MTL含量显著升高,5-HT和SS含量显著降低(P<0.05);逍遥汤组和逍遥胶囊1.62 g.kg-1组大鼠胃肠组织MTL表达的平均吸光度明显增多,SS表达明显减少,差异有统计学意义(P<0.05)。结论:逍遥方3种制剂均具有疏肝健脾作用,逍遥汤药效优于等剂量逍遥胶囊,逍遥胶囊药效则优于等剂量逍遥丸。

Objective： To compare the effect of three preparations of Xiaoyao formula （Xiaoyao capsule, Xiaoyao pill and Xiaoyao decoction） on soothing liver and strengthening spleen. Method： The liver depression and spleen deficiency rat model were established by forcing swimming, confining movement test and feeding on alternate days. The rats were randomly divided into eight groups, namely the blank group, the model group, the Cisapride group （2.7 mg·kg-1）, the Xiaoyao decoction group （1.62 g·kg-1）, the Xiaoyao pill group （1.62 g·kg-1） and the Xiaoyao capsule groups of high dose （3.24 g·kg-1）, medium dose （1.62 g·kg-1） and low dose （0.81 g·kg-1）, with intragastric administration for 3 weeks. The contents of NE, DA, 5-HT in serum were measured by HPLC-electrochemical detection method. The contents of motilin （MTL） and somatostation （SS） in plasma was determined by radioimmunassay. The expressions of MTL and SS in tissue were determined by immunohistochemical method. Result： The contents of NE and MTL in the Xiaoyao decoction group was significantly higher than that in the model group, while the content of 5-HT and SS was significantly lower than that in the model group （P〈0.01）. The expression of MTL in the Xiaoyao capsule group （1.62 g·kg-1） was significantly higher than that in the model group and the contents of 5-HT and SS was significantly lower than that in the model group （P〈0.05）. The mean optical density of MTL in gastrointestinal tissue of rats in the Xiaoyao decoction group and the Xiaoyao capsule group （1.62 g·kg-1） was remarkably higher than that in the model group, while the expression of SS was notably lower than that in the model group （P〈0.05）. Conclusion： All of the three preparations of Xiaoyao formula have the effect on soothing liver and strengthening spleen. Xiaoyao decoction shows better effect than Xiaoyao capsule with same dosage, while Xiaoyao capsule shows better effect than Xiaoyao pill with same dosage.