摘要
目的:建立青蒿素大鼠体内药物浓度的分析方法。方法:采用高效液相色谱-质谱联用技术,选用艾司唑仑为内标,样品与内标使用甲基叔丁基醚提取,并分别于m/z305,296进行测定。结果:在5～500μg.L-1,青蒿素峰面积与内标峰面积比值与浓度有良好的线性关系,最低定量质量浓度为5μg.L-1。结论:方法学证明该法能够满足青蒿素大鼠体内血药浓度的测定,可用于动物体内药物动力学的研究需求。
Objective： To develop a method for determining artemisinin in rat plasma in vivo. Method： HPLC^MS was adopt- ed. Estazolam was selected as an internal standard （ I. S. ）. The sample and I. S. were extracted using methyl tertbutyl ether and meas- ured at m/z of 305 and 296, respectively. Result ： Within the linear range of 5-500 μg · L-1, the ratio of artemisinin＇s peak area and I.S. peak area and the concentration showed good linearity, thus the minimum concentration was set to be 5 μg · L-1. Conclusion： The methodology proved that the method is so suitable for determining the drug concentration in rat blood that it can be used for stud- ying pharmacokinetics in animals.
出处
《中国中药杂志》
CAS
CSCD
北大核心
2012年第19期2964-2967,共4页
China Journal of Chinese Materia Medica
