摘要
目的:比较密闭微波辅助提取法(PMAE)、聚焦微波辅助提取法(FMAE)及微波辐射-溶剂回流提取法(PMIRE)3种微波辅助方法用于提取苦参生物碱的差异,探讨PMAE,FMAE,PMIRE的提取过程及其微波作用机制。方法:以苦参碱与氧化苦参碱为目标物,采用HPLC测定,通过优化微波功率、提取温度、提取时间、药材颗粒度、固液比等试验因素,计算其热力学函数,电子扫描电镜观察药材表面结构变化。结果:与常规的回流提取(SRE)法相比,PMAE,FMAE,PMIRE的提取效率明显提高,其热力学函数ΔH°和ΔS°明显增大,ΔG°更小。微波作用能使药材表面结构发生细胞破壁,强化提取过程。结论:微波辅助提取苦参生物碱的提取效率均优于常规的溶剂回流提取法;微波辅助的方式、作用程度、提取过程不同,提取效率有较大差异;PMAE提取时间最短;PMIRE由于微波直接作用强,细胞破壁效果更好,提取率最高,且仪器设备简单,操作简便。
Objective: To compare differences of pressurized microwave-assisted extraction method (PMAE), focused microwave-assisted extraction method (FMAE) and post microwave irradiated-reflux extraction method (PMIRE) in extracting alkaloids from Sophoraflavescens, and investigate extraction process and microwave mechanism of these 3 kinds of microwave assisted extraction methods. Method: With oxymatrine and matrine as target alkaloids, which were determined by HPLC, thermodynamic functions were calculated by optimizing microwave power, extraction temperature, extraction time, solid-liquid ratio, medicinal granule and other factors, surface structural change of herbs was observed by electronic scanning inicroscope. Result: Compared with conventional solvent reflux extraction (SRE), extraction efficiency of PMAE, FMAE and PMIRE increased significantly, AH^and AS^of alkaloids increased obviously, but AG^became smaller. Microwave irradiation could enable cell wall of herbs surface structure to rupture, and strengthen extraction process. Conclusion: Extraction efficiency of alkaloids from S. flavescens with microwave assisted extraction was superior to SRE; Extraction efficiency had large differences by different microwave assisted manners, irradiation degree and extraction process; PMAE had shortest extraction time in the closed system; Because of strong microwave direct role, PMIRE had the best cell-rupture and extraction yield, and its equipment was simple and easy-operating.
出处
《中国实验方剂学杂志》
CAS
北大核心
2012年第20期34-38,共5页
Chinese Journal of Experimental Traditional Medical Formulae
基金
广州市科技支撑计划项目(2008Z1-E301)
广东药学院师资队伍建设基金项目(52104109)
关键词
微波辅助提取
苦参
生物碱
高效液相色谱法
microwave-assisted extraction
comparative study
Sophora flavescens
alkaloids
HPLC