摘要
目的:建立HPLC测定β-榄香烯纳米粒冻干针剂中主药含量的测定方法。方法:采用高效液相色谱法,以甲醇-水(95∶5)为流动相,柱温室温,检测波长210 nm。结果:β-榄香烯在0.224～1.792μg呈良好线性关系(r=0.999 6),平均加样回收率为97.0%,RSD为1.3%。结论:本法简便、灵敏、准确、重复性好,可用于β-榄香烯纳米粒冻干针剂中β-榄香烯的含量测定。
Objective： To develop a HPLC method for the simultaneous determination of β-elemene in lyophilized powder for injection of β-elemene-polybutylcyanoacrylate nanoparticles. Method： The analysis was performed on DiamonsilTMCls （4.6 mm x 200 ram, 5 p,m）. The mobile phase was MeOH-water （95： 5） , The wavelength of the detector was set at 210 nm. Result： β-Elemene presented a good linear correlation in range of 0. 224-1. 792 ixg （r =0. 999 6）. The average recovery was 97.0% （RSD 1.3% ）. Conclusion： The method is simple, rapid and accurate, and suitable for the quality control of lyophilized powder for injection of β-ELE-PBCA- NP.
出处
《中国实验方剂学杂志》
CAS
北大核心
2012年第20期64-66,共3页
Chinese Journal of Experimental Traditional Medical Formulae
基金
辽宁省教育厅2009年度高等学校科研究项目(2009A499)
辽宁中医药大学优秀青年药学人才基金(yxrc0910)
关键词
高效液相色谱法
β-榄香烯纳米粒
冻干针剂
Β-榄香烯
HPLC
β-elemene-polybutylcyanoacrylate nanoparticles
lyophilized powder for injection
β-elemene
