摘要
目的:建立陈皮-枳壳药对提取物中柚皮苷、橙皮苷和新橙皮苷的含量测定方法。方法:采用HPLC,AgilentHypersill C18(4.0 mm×250 mm,5μm)色谱柱,以乙腈-0.1%磷酸(24∶76)为流动相,流速1 mL.min-1,检测波长283 nm,柱温40℃。结果:柚皮苷、橙皮苷和新橙皮分别在6.05~60.5,4.34~43.4,7.14~71.40 mg.L-1线性关系良好(r均>0.999 5);加样回收率分别为98.65%,98.66%,100.78%,其RSD分别为2.01%,1.52%,2.11%;重复性RSD分别为2.41%,1.56%,2.52%(n=6)。结论:该方法简便可行,重复性好,结果准确可靠,可用于陈皮-枳壳药对提取物中柚皮苷、橙皮苷和新橙皮苷的含量测定。
Objective: To develop a method to determine naringin, hesperidin and neohesperidin in Citri Reticulatae Pericarpium and Aurantii Fructus. Method: HPLC was used to determine directly naringin, hesperidin and neohesperidin of Citri Reticulatae Pericarpium and Aurantii Fructus. Phenomsil Cls (4.0 mm x 250 ram, 5 Ixm) as the chromatographic column, and acetonitrile-10% aqueous phosphoric acid (24:76)as the mobile phase, the flow rate of 1.0 mL. rain-l, the column temperature was at 40 ~C. Result: The linearity of verbascoside was good in the range of 6.05-60.5, 4. 34-43.4 and 7. 14-71.40 mg ~ L-1 ( r 〉 0. 999 5 ). The recovery of the added sample was 98.65% , 98.66% and 100.78% with RSD of 2.01% , 1.52% and 2. 11%. Conclusion: The method is simple, accurate and can be used for the determination of naringin, hesperidin and neohesperidin in Tangerine peel and Aurantii Fructus extract.
出处
《中国实验方剂学杂志》
CAS
北大核心
2012年第20期100-103,共4页
Chinese Journal of Experimental Traditional Medical Formulae