HPLC测定陈皮-枳壳药对提取物中柚皮苷、橙皮苷和新橙皮苷的含量

HPLC Determination of Naringin,Hesperidin and Neohesperidin in Citri Reticulatae Pericarpium and Aurantii Fructus Extract

目的:建立陈皮-枳壳药对提取物中柚皮苷、橙皮苷和新橙皮苷的含量测定方法。方法:采用HPLC,AgilentHypersill C18(4.0 mm×250 mm,5μm)色谱柱,以乙腈-0.1%磷酸(24∶76)为流动相,流速1 mL.min-1,检测波长283 nm,柱温40℃。结果:柚皮苷、橙皮苷和新橙皮分别在6.05～60.5,4.34～43.4,7.14～71.40 mg.L-1线性关系良好(r均>0.999 5);加样回收率分别为98.65%,98.66%,100.78%,其RSD分别为2.01%,1.52%,2.11%;重复性RSD分别为2.41%,1.56%,2.52%(n=6)。结论:该方法简便可行,重复性好,结果准确可靠,可用于陈皮-枳壳药对提取物中柚皮苷、橙皮苷和新橙皮苷的含量测定。

Objective： To develop a method to determine naringin, hesperidin and neohesperidin in Citri Reticulatae Pericarpium and Aurantii Fructus. Method： HPLC was used to determine directly naringin, hesperidin and neohesperidin of Citri Reticulatae Pericarpium and Aurantii Fructus. Phenomsil Cls （4.0 mm x 250 ram, 5 Ixm） as the chromatographic column, and acetonitrile-10% aqueous phosphoric acid （24：76）as the mobile phase, the flow rate of 1.0 mL. rain-l, the column temperature was at 40 ~C. Result： The linearity of verbascoside was good in the range of 6.05-60.5, 4. 34-43.4 and 7. 14-71.40 mg ~ L-1 （ r 〉 0. 999 5 ）. The recovery of the added sample was 98.65% , 98.66% and 100.78% with RSD of 2.01% , 1.52% and 2. 11%. Conclusion： The method is simple, accurate and can be used for the determination of naringin, hesperidin and neohesperidin in Tangerine peel and Aurantii Fructus extract.