青娥丸质量标准研究——君药杜仲的含量测定

Quality Standard for Qing'e Wan——the Quantitative Methods of Eucommiae Cortex

目的:制定复方青娥丸的质量标准。方法:采用HPLC对处方中松脂醇二葡萄糖苷(PDG)含量进行定量测定。结果:HPLC法测定松脂醇二葡萄糖苷在4.43～443 mg.L-1线性关系良好,回归方程为Y=28 849X-2 119.5(r=0.999 9),平均加样回收率为100.99%(RSD 2.17%),符合分析要求。结论:所建立定量检测方法操作简便、准确可靠、灵敏度高、专属性强,可有效控制青娥丸的质量。

Objective： To establish the quality standard for Qing＇e Wan. Method： （ ＋ ） -Pinoresinol di-O-13-D-glucopyranoside （PDG） was determined by HPLC. Result： The results of quantitative evaluation showed that （ ＋ ） -pinoresinol di-O-fl-D-glucopyranoside （PDG） concentration was linear in the range of 4.43-443 mg· L-1 （r=0.999 9）, the average recovery rate was 100.99% （RSD 2. 17%）. This method met the analysis requirement. Conclusion： The established quantitative methods were simple, accurate, reliable and reproducible, which can be effectively used for the quality control of Qing＇e Wan.