金花茶叶乙醚部位的GC-MS分析

GC-MS Analysis of Ether Extraction of Camellia chrysantha

目的:采用气相-质谱(GC-MS)分析金花茶叶乙醚部位的化学成分。方法:采用75%乙醇回流提取金花茶叶,滤液浓缩后用乙醚萃取,得到乙醚部位,用GC-MS法分析鉴定其化学成分,结合计算机检索技术及美国国家标准技术研究所(NIST)化合物谱库检索进行鉴定,再用面积归一化法确定其相对含量。结果:金花茶叶乙醚部位的成分共分离鉴定出61个化合物,相对含量占总含量的78.41%,主要化学成分是2-(氧代十八烷基)乙醇,二十五烷,1-碘代十六烷等。结论:可用GC-MS法快捷、简便、准确地分析金花茶叶乙醚部位成分。初步揭示了该药材化学成分,对进一步研究其化学成分、为药材的质量评价提供实验基础。

Objective： To analyze the chemical constituents of volatile oil from the leaves of Camellia chrysantha. Method： By extracting C. chrysantha with 75% ethanol, the filtrate was concentrated and extracted with ether to get the ether parts, and the chemical composition was identified and analyzed by GC-MS method. Combining with computer retrieval technology and search of national institute of standards and technology （NIST） compound spectral-library for identification, their relative content was determined by the area normalization method. Result： Sixty-one kinds of chemical constituents were isolated from the ether parts of C. chrysantha. The relative content is 78.41% in the total content, the main chemical ingredients were 2-（oxo-18 alkyl） ethanol, two pentadecane, 1-iodo-hexadecane, and so on. Conclusion： The present GC-MS method is fast, simple and accurate to analyze the composition of ether parts of C. chrysantha. It initially revealed the chemical composition of the medicine, and provided the experimental basis for further study of its chemical composition and the medicinal quality evaluation.