脑脉舒康胶囊对短暂性脑缺血模型小鼠的影响

Experimental Study of Naomai Shukang Capsule on Transient Ischemic Attack in Mice

目的:探讨脑脉舒康胶囊对短暂性脑缺血发作小鼠模型的影响。方法:采用昆明种小鼠,分为脑脉舒康胶囊组(1.8,0.9,0.45 g.kg-1)、模型组、假手术和阳性药(血栓通1.125 g.kg-1、尼莫地平0.03 g.kg-1)对照组,各组连续灌胃7 d,于第8天给药1 h后,结扎两侧颈总动脉30 min,并进行再灌注15 min制备短暂性脑缺血模型;眼球取血,测定超氧化物歧化酶(SOD)活性、丙二醛(MDA)含量;取部分小鼠脑组织匀浆,检测脑匀浆液Na+-K+-三磷酸腺苷酶(ATPase),Mg2+-ATPase,Ca2+Mg2+-ATP,Ca2+-ATPase活力;部分脑组织称重后计算脑含水量。结果:脑脉舒康胶囊可以增强Na+-K+-ATPase,Mg2+-ATPase,Ca2+-Mg2+-ATP,Ca2+-ATPase活力,降低MDA含量,提高SOD的活性,减轻脑缺血引起的脑组织间质水肿。结论:脑脉舒康胶囊可提高脑组织ATP能量,稳定离子泵功能,清除氧自由基,缓解脑水肿,具有保护脑细胞的作用。

Objective： To study the effects of Naomai Shukang capsule on transient ischemic attack in mice. Method： Kunming mice were divided into Naomai Shukang capsule group （ 1.8, 0.9, 0.45 g kg-1） , the model group, sham operation control group, and the positive control group （Xueshuantong 1. 125 g-kg-1, nimodipine 0.03 g kg-1 ） , mice were fed Naomai Shukang capsule for 7 d, on the 8 d, after 1 h of administration, both sides of the common carotid artery were ligatured for 30 min and reperfusion for 15 min to make transien cerebral ischemia model; superoxide dismutase （SOD） and malonaldehyde （MDA） content were determinated; section of brain tissue was homogenated, Na ＋-K ＋-adenosine triphosphatase （ATPase） , Mg2 ＋-ATPase, Ca2 ＋- Mg2＋-ATP, Ca2＋-ATPase activity of brain homogenate were measured, part of brain tissue was calculated after weighing for brain water content. Result： Naomai Shukang capsule could enhance Na^-K^-ATPase, Mg2＋- ATPase, Ca2＋ -Mg2＋-ATP, Ca2＋-ATPase activity in brain, and decrease MDA content, increase SOD activity, brain edema was relieve induced by cerebral ischemia. Conclusion： Naomai Shukang capsule can improve ATP energy of brain, scaveng oxygen free radicals, relieve cerebral edema, protect brain cells, reduce brain structure and function damage.