摘要
目的:观察大鼠胃体、胃底离体平滑肌条自发性收缩及电场刺激(EFS)、乙酰胆碱(ACh)和A型肉毒素(BTX-A)对肌条收缩的影响,并探讨其机制。方法:取大鼠胃体胃底平滑肌制备肌条,肌条随机分为对照组、EFS组、BTX-A(10 U.mL-1)组、BTX-A(10 U.mL-1)+EFS组、ACh(100μmol.L-1)组、ACh(100μmol.L-1)+BTX-A(10 U.mL-1)组、ACh(100μmol.L-1)+阿托品(1μmol.L-1)组,采用Biolap 420E生物机能实验系统记录肌条收缩数据。结果:EFS引发胃体平滑肌张力增强(P<0.05)及振幅增大(P<0.01),引发胃底平滑肌张力增强(P<0.01)及振幅增大(P<0.05);BTX-A降低胃体平滑肌自发性收缩张力及振幅(P<0.01),引发胃底平滑肌自发性收缩张力下降(P<0.05);BTX-A可抑制EFS引发的胃体平滑肌张力增强及振幅增大效应(P<0.01),对EFS引发的胃底平滑肌张力增强(P<0.01)及振幅增大(P<0.05)也产生抑制;BTX-A降低ACh引发的胃体胃底平滑肌收缩张力增强及振幅增大(P<0.01)。结论:EFS可增强胃体胃底平滑肌收缩能力;EFS对BTX-A作用后胃体胃底平滑肌收缩不产生增强作用;BTX-A可抑制胃体胃底平滑肌自发性收缩及ACh引发的收缩。
Objective: To observe the effect of botulinum toxin type A (BTX-A), electrical field stimulation (EFS) and acetylcholine (ACh) on .spontaneous contractility in gastric body and gastric fundus smooth muscle. Method: Muscle strips in gastric body and gastric fundus were prepared, and subdivided randomly into control group, EFS group, BTX-A (10 U "mL-1) group, BTX-A (10 U "mL-1) + EFS group, ACh (100 ixmol'L-1) group, ACh (100 ixmol.L-j) +BTX-A (10 U ~ mL-j) group, Ach (I00 Ixmol ~ L-j) + Atropine (1 p^mol .L-l) group. The data were recorded by physiological experimental system of BL-420. Result: EFS enhanced the tension (P 〈 0.05) and amplitude (P 〈 0.01 ) in gastric body contractility, and similar results was observed in gastric fundus contractility; BTX-A decreased spontaneous contractile tension and amplitude (P 〈0.01) in gastric body and tension (P 〈 0.05) in gastric fundus; BTX-A inhibited EFS-induced smooth muscle contractility including tension and amplitude (P 〈 0.01 ) in gastric body, tension (P 〈 0.01 ) and amplitude (P 〈 0.05) in gastric fundus, BTX-A inhibited ACh-induced smooth muscle contractility including tension and amplitude (P 〈0.01) in gastric body and gastric fundus. Conclusion: EFS enhances smooth muscle spontaneous contractility in gastric body and gastric fundus; BTX-A inhibits gastric body and gastric fundus smooth muscle spontaneous contractility; BTX-A inhibits EFS and ACh-induced smooth muscle contractility in gastric body and gastric fundus.
出处
《中国实验方剂学杂志》
CAS
北大核心
2012年第20期240-243,共4页
Chinese Journal of Experimental Traditional Medical Formulae
基金
河南省教育厅自然科学研究项目(2011C310012)
兰州大学医学基金项目(LZUYX200605)
关键词
A型肉毒素
胃体
胃底
乙酰胆碱
电场刺激
botulinum toxin A
gastric body
gastric fundus
acetylcholine
electrical field stimulation