平胃散水煎液对湿困脾胃证大鼠疲劳状态的改善作用

Antifatigue Effect of Pingwai San on Rats of the Spleen and Stomach being Stranded by Dampness

目的:探讨平胃散对湿困脾胃证大鼠疲劳状态的改善作用及其机制。方法:将大鼠随机分为空白对照组,模型组,平胃散高、中、低剂量组。造模第8天平胃散各剂量组分别灌胃平胃散水提液1.5,3,6 g.kg-1。空白对照组、模型组灌胃等量生理盐水,给药7 d后观测大鼠力竭游泳时间;检测血浆乳酸(LA)、血清乳酸脱氢酶(SLDH)、血清尿素氮(SUN)、肝糖原含量、血红蛋白(Hb)的含量。结果:平胃散高、中、低剂量组均能不同程度地改善模型大鼠的行为学表现,提高大鼠力竭游泳时间。与模型组比较,高剂量组肝糖原含量显著增高(P<0.01),高、中剂量组SUN含量显著降低(P<0.05)。各组大鼠Hb,LA,SLDH水平未见明显差异。结论:平胃散改善湿困脾胃证疲劳状态的作用可能与减少在运动负荷下的蛋白质与氨基酸的分解,提高肝糖原的含量有关。

Objective： To study the antifatigue effect of Pingwei San （PWS） on rats of the spleen and stomach being stranded by dampness. Method： Rat models of the spleen and stomach being stranded by dampness was made. The rats were divided randomly into five groups： the control group, the model group and three groups administered with high, mediate and low dose of respectively, at the eighth day after modeling, the control group and the model group were administrated with physiological saline solution, the others three groups were administered with 1.5, 3, 6g .kg-1 PRFS respectively. After final administrating, the behavioral changes of rats were observed and the exhaustive swimming time of rats was recorded. At the end of the experiment, the levels of blood plasma lactic acid （ LA）, serum lactate dehydrogenass （ SLDH ）, serum urea nitrogen （ SUN）, hepatic glycogen, and hemoglobin （Hb） were determined. Result： The rat behaviors in the three groups with PWS were remarkably improved. The last exhaustive swimming time increased significantly, hepatic glycogen level of high- dose group rats was obviously increased （P 〈 0.01 ）, and SUN level of high-dose and mediate-dose group rats were decreased significantly （P 〈 0.05）. The levels of Hb, LA and SLDH in all animals were not different obviously. Conclusion： Decreasing disintegration of protein and amino acids, and increasing the level of hepatic glycogen in exercise load were possibly antifatigue mechanism of PWS.