葫芦茶不同提取物对链脲佐菌素致糖尿病小鼠的影响

Effects of Extracts from Tadehagi triquetrum in Diabetic Mice Induced by Streptozotocin

目的:研究葫芦茶不同提取物对链脲佐菌素(STZ)所致糖尿病小鼠血糖(BG)、血脂及胰岛素(INS)的影响。方法:采用系统溶剂回流提取法对葫芦茶进行提取分离,分别得到石油醚部位、氯仿部位、乙酸乙酯部位、正丁醇部位、60%乙醇部位,采用腹腔注射STZ 150 mg.kg-1建立2型糖尿病小鼠模型,将实验小鼠随机分为8组:空白组,模型组,二甲双胍组(100mg.kg-1),石油醚部位组、氯仿部位组、乙酸乙酯部位组、正丁醇部位组、60%乙醇部位组,给药剂量均为200 mg.kg-1,空白组和模型组给予等剂量的生理盐水,每天灌胃给药1次,连续灌胃给药20 d后,眼球取血,采用葡萄糖氧化酶法测定各组小鼠空腹血糖(FBG),放射免疫法测定空腹胰岛素(FINS),生化法检测血脂。结果:与模型组比较,乙酸乙酯部位、正丁醇部位、60%乙醇部位对STZ所致DM小鼠有显著降低FBG的作用(P<0.05或P<0.01),其中60%乙醇部位降糖效果最为显著,降糖率为23.61%,能降低胰岛素抵抗指数(IR)及甘油三酯(TG)、总胆固醇(TC)、低密度脂蛋白胆固醇(LDL-C),显著提高胰岛素敏感指数(ISI)和高密度脂蛋白胆固醇(HDL-C)(P<0.05或P<0.01),石油醚部位、氯仿部位对DM小鼠无明显影响(P>0.05)。结论:葫芦茶降血糖有效成分主要存在于正丁醇部位、60%乙醇部位,能明显降低STZ致DM小鼠模型的BG水平,改善胰岛素抵抗,调节脂代谢紊乱的作用。

Objective： To study the effects of extracts from T. triquetrum on blood glucose and blood lipid and insulin （INS） in diabetic mice induced by streptozotocin. Method： T. triquetrum was extracted and separated by reflux extraction with different solvent, petroleum ether fraction, chloroform fraction, ethyl acetate fraction, n- butanol fraction and 60% ethanol fraction were obtained respectively. Type 2 diabete mice models were established by intraperitoneal injection of streptozotocin （STZ） 150 mg ＂kg-1. The experimental mice were randomly divided into eight groups, control group, model group, metformin group （100 mg＂ kg-1） , petroleum ether group, chloroform group, ethyl acetate group, n-butanol group and 60% ethanol group, the dose were 200 mg kg-1. Control group and model group were given normal saline. After the experimental mice were administrated with drugs for 20 days, the fasting blood glucose was determined by method of glucose oxidase, fasting insulin was determined by the radioimmunoassay, and blood lipid were determined by chemical method. Result： Compared to model group, ethyl acetate fraction, n-butanol fraction and 60% ethanol fraction could significantly reduce fasting blood glucose level in diabetic mice induced by STZ （P 〈 0.05 or P 〈 0.01 ）. Of all the fractions, 60% ethanol parts had the most significant hypoglycemic effect, hypoglycemic rate was 23.61%. It also decreased insulin resistance index （IR）, contents of TG, TC and LDL-C and increased the insulin sensitivity index （ISI） and HDL-C content in the serum significantly （ P 〈 0.05 or P 〈 0.01 ）. Chloroform fraction and petroleum ether fraction had no significant effect on diabetic mice （P 〉 0. 05 ）. Conclusion： The effective constituent of antidiabetics from Tadehagi triquetrum are mainly exist in the n-butanol fraction and 60% ethanol fraction, they can significantly reduce fasting blood glucose level in diabetic mice induced by STZ, improve insulin resistance and adjust metabolic disturbance.