二氧化硫对实验性腹主动脉-下腔静脉分流大鼠肺动脉压力的影响

Effect of Sulfur Dioxide on Pulmonary Artery Pressure in Rats with Experimental Abdominal Aorta and Inferior Vena Cava Shunting

目的研究二氧化硫(SO2)对高肺血流性肺动脉高压的调节作用。方法 25只雄性Sprague Dawley大鼠随机分为对照组(n=8)、分流组(n=8)和分流+SO2供体组(n=9)。对分流组和分流+SO2供体组大鼠行腹主动脉-下腔静脉分流术;分流+SO2供体组大鼠每天腹腔内注射亚硫酸钠/亚硫酸氢钠(Na2SO3/NaHSO3)85 mg·kg-1·d-1,8周后以右心导管的方法测定3组大鼠肺动脉收缩压(PASP)、肺动脉平均压(PAMP)及肺动脉舒张压(PADP)。结果分流组大鼠PASP显著高于对照组大鼠[(3.91±0.66)kPa vs(2.26±0.47)kPa,P<0.05];分流+SO2供体组大鼠PASP显著低于分流组大鼠[(3.04±0.36)kPa vs(3.91±0.66)kPa,P<0.05];分流+SO2供体组大鼠PASP较对照组大鼠[(3.04±0.36)kPa vs(2.26±0.47)kPa,P<0.05]显著增高。分流组大鼠PADP较对照组大鼠显著升高[(1.81±0.45)kPa vs(1.26±0.32)kPa,P<0.05];而分流+SO2供体组大鼠PADP与分流组大鼠比较差异无统计学意义[(1.49±0.19)kPa vs(1.81±0.45)kPa,P>0.05]。分流组大鼠PAMP显著高于对照组大鼠[(2.53±0.43)kPa vs(1.60±0.36)kPa,P<0.05];分流+SO2供体组大鼠PAMP显著低于分流组大鼠[(2.01±0.23)kPa vs(2.53±0.43)kPa,P<0.05]。分流+SO2组PAMP显著高于对照组[(2.01±0.23)kPa vs(1.60±0.36)kPa,P<0.05]。结论 SO2可明显降低高肺血流大鼠的PASP和PAMP。

Objective To explore the effect of sulfur dioxide （ SO2 ） on pulmonary artery pressure of rats with abdominal aorta and infe- rior vena cava shunting surgery with the help of detection of pulmonary artery systolic pressure （PASP） pulmonary artery, mean pressure （PAMP） and pulmonary artery diastolic pressure （PADP）. Methods To explore the effect of SO2 on pulmonary artery pressure of rats with abdominal aorta and inferior vena cava shunting surgery with the help of detection of PASP, PAMP and PADP. A total of 25 male Sprague Daw- ley rats were divided randomly into the following groups ： control group, shunting group and shunting plus SO2 donor group. For rats of shunting group and shunting plus SO2 group, an abdominal aorta and inferior vena cava shunting was created. Na2 SO3/NaHSO3 was administrated intra- peritoneally to rats of shunting plus SO2 group. After 8 - week shunting,PASP ,PAMP and PADP of each rats were evaluated by using a right cardiac catheterization procedure. Results Compared with control group, the PASP of rats in shunting group was increased significantly [ （ 3.91 ± 0.66） kPa vs （ 2.26 ± 0.47 ） kPa,P 〈 0.05 ] ; Compared with shunting group, the PASP of rats in shunting plus SO2 donor group was decreased significantly [ （3.04 ± 0.36） kPa vs （ 3.91 ± 0.66） kPa,P 〈 0.05 ] ; The PASP of rats in shunting plus SO2 donor group was higher than that of rats in control group [ （3.04 ±0.36） kPa vs （2.26 ±0.47） kPa,P〈0.05]. Compared with control group, the PADP of rats in shunting group was increased significantly [ （1.81 ± 0.45 ） kPa vs （1.26 ± 0.32） kPa,P 〈 0.05 ] ; There was no difference between the PADP of rats in the shunting group and shunting plus SO2 donor group [ （ 1.49 ± 0.19） kPa vs （ 1.81 ± 0.45 ） kPa,P 〉 0.05 ]. Compared with control group, the PAMP of rats in shunting group was increased significantly [ （ 2.53 ± 0.43 ） kPa vs （ 1.60 ± 0.36 ） kPa, P 〈 0.05 ] ; Compared with shunting group, the PAMP of rats in shunting plus SO2 donor group was decreased significantly [ （2.01 ± 0.23） kPa vs （2.53 ± 0.43 ） kPa ,P 〈 0.05 ] ; The PAMP of rats in shunting plus SO2 donor group was higher than that of rats in control group [ （2.01 ± 0. 23 ） kPa vs （ 1.60 ± 0.36） kPa, P 〈 0.05 ]. Conclusions SO2 obviously decreases the PAMP and PASP of rats with abdominal aorta and inferior vena cava shunting.