慢性阻塞性肺疾病患者呼出气冷凝液中NO2-、LTB4、PGE2、IL-6、IL-10水平的研究

Levels of NO2-, LTB4, PGE2, IL-6, IL-10 in exhaled breath condensate with acute exacerbation chronicobstructive pulmonary disease patients

目的探讨慢性阻塞性肺疾病（COPD）患者呼出气冷凝液（EBC）中亚硝酸盐（NO2-）、白三烯B4（LTB4）、前列腺素E2（PGE2）、白介素6（IL-6）和IL-10的水平与气道炎症及肺功能的关系。方法收集20例COPD患者和20名健康体检者，测定肺通气功能，收集EBC，用比色法测定EBC中NO2-水平，用ELISA法测定EBC中LTB4、PGE2、IL6和IL-10的水平。结果①COPD组EBC中NO2-和LTB4的水平分别为（2．029±1.992）μmol／L、（0．400±0．235）μmol／L，均显著高于健康对照组（0．400±0．235）μmol／L，（9．742±2．348）ng／L，差异有统计学意义（P值均〈0．05）；EBC中PGE2、IL-6、IL-10的水平在两组间比较，差异均无统计学意义（P〉0．05）；COPD组EBC中NO2-、LTB4、PGE2、IL6、IL-10的水平与FEV，／FVC和FEV，％预计值无相关性（P〉0．05）。结论COPD患者EBC中NO2-、LTB4与气道炎症及氧化应激有关系，PGE2、IL-6、IL-10与气道炎症的关系需进一步研究，这些细胞因子与肺功能无相关性。

Objective To investigate the relationship between NO2-, LTB4, PGE2, IL-6 and IL 10 levels in exhaled breath condensate （EBC） and airway inflammation as well as pulmonary function from COPD patients. Methods Twenty cases of COPD and twenty healthy control were enrolled. ALL subjects detected FEV1/FVC and FEV1 % predict. EBC were collected using EeoScreen system. The NO2 level in EBC was determined by the spectrophotometry. The levels of LTB4, PGE2 , IL-6 and IL-10 in EBC were measured by enzyme linked immuosorbent assay. Results The levels of NO2 and LTB4 in EBC of COPD group were significantly higher than that of the control group [（2. 029±1. 992） μmol/L, （13. 598±3. 910） ng/L vs （0. 400±0. 235） μmol/L,（9. 742±2. 348） ng/L,respectively, all P 〈0. 052. There were no significant difference of PGE2 , IL 6 and IL-10 levels in EBC both groups （all P 〉0.05）. There were no correlation between NO2 , LTB4, PGE2, IL-6, IL-10 in EBC with FEV1/FVC and FEV1% predict （ P 〉0.05）. Conclusions The COPD patients NO2 and LTB4 levels in EBC are related to airway inflammation and oxidative stress. Whether PGE2, IL-6, IL-10 reflecting airway inflammation need further study. All of cytokines were failed to correlate with Lung functiom.