蜈蚣及蚯蚓的可溶性蛋白质SDS-PAGE图谱研究

Studies on Sodium Dodecyl Sulphate-polyacylamide Gel Electrophoresis is atlas of protein in scolopendra and pheretima

目的为常见动物类中药材的真伪鉴别及其蛋白质的研究提供科学依据。方法用SDS-PAGE测定蜈蚣及蚯蚓的主要蛋白质分子量。结果获得清晰的蜈蚣和蚯蚓的电泳谱带及相应数据。结论操作简单,方法准确,重复性好,可靠方便,为蜈蚣和蚯蚓等动物类药材及其制剂的生产、质量控制提供有价值的参考数据和图谱。

Objective To offer scientific basis of distinguish animal TCM and studies on their protein. Methods Molecular weights of the proteinswere measured by modified sodium dodecyl sulphate-polyaerylamide gel electrophpresis （SDS-PAGE）. Results Get clear centipede and earthworm electrophoresis bands and corresponding data. ConclusionThe mothod is accurate, reliable and with good reproducibility. It can supply important reference datas and atlas on quality control and preparation produce of Scolopendra and Pheretima