内脂素基因启动子-1535C/T多态性与子痫前期发病的关系

Quantitative study of the-1535 promoter variant of the visfatin gene is associated with preeclampsia

目的探讨子痫前期患者内脂素基因启动子-1535C/T多态性与子痫前期发病的关系。方法选择2010年7月至2011年1月福建医科大学附属第二医院收治的96例子痫前期孕妇,其中轻度子痫前期35例和重度子痫前期61例;96例同期正常晚期妊娠孕妇;采用ELISA及Multiplex SNaPshotSNP分型法分别检测血清内脂素水平、内脂素基因启动子-1535位点基因型。结果子痫前期组孕妇血清内脂素水平明显高于对照组,重度子痫前期组高于轻度子痫前期组,差异有统计学意义(P<0.05)。内脂素基因启动子-1535位点基因型和等位基因频率在子痫前期组和对照组中的分布差异有统计学意义(P<0.05)。子痫前期组中,内脂素基因启动子-1535CC型比TT型血清甘油三酯(TG)、内脂素(visfatin)水平明显升高,高密度脂蛋白(HDL-C)明显降低(P<0.01)。结论子痫前期患者内脂素基因启动子-1535CC型与血清内脂素水平、血脂水平相关,参与子痫前期的发病。

Objective To detect the -1535 promoter variant of the visfatin gene is associated with preeclampsia. Methods A total of 96 women with preeclampsia cases and 96 normal pregnant women controls participated in this study. All are admitted to my hospital for delivery between July 2010 and January 2011. Patients with PE were divided into severe PE group（n =61 ） and mild PE group （n = 35 ）. ELISA was used to detect the levels of visfatin. Multiplex SNaPshotSNP typing method used assay maternal visfatin gene promoter-1535, determine the genotypes. Results The level of serum visfatin in PE group significantly elevated as in control group, the level in severe PE group significantly elevated as in mild PE group. The distribution of visfatin of genetype and allele frequencies of the SNP-1535 were different in obesity and normal control group（P 〈0.05）. In PE group,the levels of TG and serum visfatin were significantly elevated in the subjects with SNP-1535CC genotype as compared to Tr genotype,and HDL-C decreased significantly（ P 〈 0. 01 ）. Con- clusions The -1535CC of the visfatin gene and serum visfatin, lipid levels relate to participation in the pathogenesis of preeclampsia.