雌激素对盆底功能障碍性疾病患者阴道壁成纤维细胞力学反应的影响

Effects of 17β-estradiol on the changes of fibroblasts derived from vaginal wall in patients with PFD exposed to mechanical stretch

目的研究体外培养盆底功能障碍性疾病(pelvic floor dysfunction,PFD)患者阴道壁成纤维细胞的力学反应及17β-雌二醇(E2)对其力学反应的影响。方法河北医科大学第二医院2009年10月至2010年9月,取12例PFD患者阴道壁组织块原代培养成纤维细胞,免疫细胞化学法鉴定,绘制生长曲线,Flexercell4000系统加载力,卸载后加入17β-E2,RT-PCR检测Ⅰ型胶原(ColⅠ)、Ⅲ型胶原(ColⅢ)、基质金属蛋白酶-1(MMP-1)和金属蛋白酶组织抑制剂-1(TIMP-1)的表达。结果 (1)应力组ColⅠ、MMP-1表达较静力组明显增强,ColⅢ、TIMP-1表达明显减弱(P<0.01)。(2)17β-E2干预后,ColⅠ、ColⅢ、TIMP-1表达较对照组增强,MMP-1表达减弱(P<0.05)。结论 (1)应力改变了体外培养成纤维细胞的胶原代谢状态。(2)17β-E2部分逆转了应力后成纤维细胞的低反应性。

Objective To study the response of vaginal wall fibroblasts obtained from patients with pelvic floor dysfunction （PFD） to mechanical stimulation, and the effect of the 17β-estradiol （E2 ） on the mechanical response. Methods Twelve patients with PFD who underwent surgery therapy in the Second Hospital of Hebei Medical University between Oc- tober 2009 and September 2010 were recruited. Fibroblasts were primarily cultured by vaginal wall tissue mass, and iden- tified by morphology and immunocytochemieal methods. Cell growth curve was drawn. Fibroblasts were loaded by Flexer- cell 4000 tension system. Unloaded fibroblasts were treated with E：. Gene expression of Col I , Col III, MMP-1 and TIMP- 1 were measured by RT-PCR. Results （ 1 ） After mechanical stretch, gene expressions of Col [ and MMP-1 were obviously higher than that of static controls, while that of Col III and TIMP-1 were obviously lower（ P 〈 0. 01 ） ; （2） Treated with Ez ,gene expressions of Col I , Col III and TIMP-1 increased than that of untreated controls, while that of MMP-1 （ P 〈 O. 05 ） decreased . Conclusions （ 1 ） Mechanical stretch changes the collagen metabolism of vaginal wall fi- broblasts. （2）17β-estradiol can reverse partially the decreased mechanical reaction of fibroblasts.