期刊文献+

海藻糖生物特性及其在ELISA技术研发中的应用 被引量:1

Biological Properties of Trehalose and its Application in ELISA
下载PDF
导出
摘要 海藻糖属非还原性双糖,在生物体内作为一种贮藏性糖类,可提供能量来源,同时它也是一种重要的应激代谢产物,对应激状态具有高度抗性。外源性海藻糖对细胞、抗体等生物活性物质同样具有非特异性生物保护作用。文章综述了海藻糖的能量储备、抗应激、细胞稳定剂等生物学特性,以及对细胞、抗体和ELISA中包板抗原或抗体的保护作用,并对其在ELISA技术改善中的应用前景做了推测与展望。 Trehalose is a non-reducing disaccharide whose function in some organisms is as a source of energy or a pro- tectant against the stress. The exogenous trehalose is contributed to protecting the bioactivitie of some substances like cells and antibodies in vitro. This review summarizes the biological properties of trehalose, such as energy source, stress resistance, cell stabilizer and helping to stabilize antigens or antibodies in ELISA. The trehalose is also helpful for improving ELISA detection.
出处 《中国畜牧兽医》 CAS 北大核心 2012年第10期69-72,共4页 China Animal Husbandry & Veterinary Medicine
基金 甘肃省科技支撑计划项目(1011NKCA048) 中央级公益性科研院所基本科研业务费专项资金项目(BRF09030)
关键词 海藻糖 生物学特性 ELISA trehalose biological properties ELISA
  • 相关文献

参考文献29

  • 1王子辉,楚杰,王君高,刘可春.布拉酵母菌海藻糖提取方法的比较研究[J].中国畜牧兽医,2010,37(7):52-54. 被引量:2
  • 2齐向辉,陈华友,蒙健宗,侯守海,梁淑华,徐虹,黄日波.海藻糖在生物组织器官保护方面的新应用[J].安徽农业科学,2009,37(33):16729-16732. 被引量:3
  • 3Alvarez-peral F J, Zaragoza O, Pedreno Y, et al. Protective role of trehalose during severe oxidative stress caused by hydrogen peroxide and the adaptive oxidative stress response in Candida al- bicans[J]. Microbiology, 2002, 148(8): 2599-2606.
  • 4Benaroudi N, Lee D H, Goldberg A L. Trehalose accumulation during cellular stress protects cells and cellular proteins from damage by oxygen radicals [J]. J Biol Chem, 2001, 276 (26):24261-24267.
  • 5Bezttie G M, Crowe J H, Lopez A D, et al. Trehalose:a cryo- protectant that enhances recovery and preserves function of hu- man pancreatic islets after long-term storage [J]. Diabetes, 1997, 46 (3): 519-523.
  • 6Blakeley D, Roser B, Tolliday B, et al. Dry instant blood typing plate for bedside use[J]. The Lancet, 1990, 336(8719) : 854-855.
  • 7Characterization and stabilization by covalent modification, and application for the synthesis of a, a-trehalose[J]. J Biotechnol, 2007, 129(1):140-150.
  • 8Chen Q, Haddad G. Role of trehalose phosphate synthase and trehalose during hypoxia: from flies to mammals[J]. J Exp Biol, 2004, 207(18):3125-3129.
  • 9Clegg J S, Evans D R. The physiology of blood trehalose and its function during flight in the blowfly[J].J Exp Biol, 1961, 38: 771-792.
  • 10Cleland J L, Lam X, Kendrick B, et al. A specific molar ratio of stabilizer to protein is required for storage stability of a lyophi- lized monoclonal antibody[J]. Journal of Pharmaceutical Sci- ences, 2001, 90: 310-321.

二级参考文献7

共引文献3

同被引文献9

引证文献1

二级引证文献2

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部