农药残留检测用植物酯酶筛选研究

Selection of Phytoesterases and Reaction Conditions for Using Plant Esterases to Detect Pesticide Residues

目的:选用4种块茎马铃薯、红薯、茨菇、芋头提取植物酯酶,研究其检测有机磷农药的最佳条件,拟选取适宜的植物酶源;方法:使用酶抑制法,研究4种块茎中提取出的植物酯酶对农药敌敌畏、敌百虫、甲胺磷、辛硫磷灵敏度及其最低检测限;结果:4种植物酯酶均对农药敌百虫的灵敏度最好,最高可达37.184,检测限也低于国家标准;结论:因此,该酶可作为新的有机磷农药残留检测用酶。

Objective ： Using Plant esterase obtain Four tuber （Solanum tuberosum L, lpomoea batatas, Sagittaria sagittifolia, Colocasia esculenta （L.） Schoot）,This article reports optimal condition for detection of Organophosphorus Pesticide Resi- due. in order to select the optimal esterase for the detection ; Method：Based on the inhibiting mechanism of enzyme, phy- toesterases from various plant sources are investigated the sensitivity and the limit of detectable to dichlorvos （DDVP） .dip- terex,trichlorphon . Methamidophos, phoxim. Results：The results showed that the limits of detection were lower than the maximum of residue limit ,which meets the national standard of detection. Conclusion：The results indicated that the esterase obtained from Ipomoea and Colocasia esculenta make an optimal enzyme source for detecting agricultural pesticides residue.