诱导子对茶条槭悬浮细胞核酸酶的激活研究

The Study of Nuclease Activated by Elicitors in Cell Suspension of Acer ginnala

为揭示真菌诱导子诱导茶条槭悬浮细胞发生凋亡的原因,以茶条槭悬浮细胞为材料,采用细胞形态观察、基因组DNA电泳分析以及核酸酶活性检测等手段,研究了真菌诱导子对细胞发生凋亡的影响。结果表明,真菌诱导子处理的细胞,其形态结构发生变化,DNA降解呈梯状的条带,且检测到分子量大约为50、40、41、38、34kDa的核酸酶。通过这些结果说明,茶条槭悬浮细胞在真菌诱导子诱导下发生细胞凋亡,并伴有特异核酸酶被激活。

The aim was to reveal the reason causing apoptosis of Acer ginnala cell co-cultured with a fungal elicitor.The microscope observation,genomic DNA electrophoresis and nuclease activity test was used to investigate the apoptosis of cell suspension.It was observed from the microscope that the shape and structure of the cells altered significantly.The genomic DNA of induced cells ran a ladder graph on the lane of gel electrophoresis and then smeared on the lane when the cells died.It was also detected that the nucleases of the cell were activated after induced.Specific bands with the size of 50,40,41,38 and 34 kDa were detected on the PAGE.The fungal elicitor activates nucleases to degrade the genomic DNA,and bring the cells to apoptosis eventually.