不同光质对红花山楂试管苗生长的影响

Different Light Qualities on Growth of Crataegus in Vitro

以红花山楂分化试管苗为试验材料,研究不同光质对红花山楂分化、生根、移栽后的生长情况的影响,以此缩短植物组培生长周期,提高组培苗生长质量,为不同光质对其他科属植物的快速繁殖打开思路,也为光质对植物的影响提供数据支撑与理论依据。结果表明,红蓝比为7:9的光质对红花山楂分化、生根影响最大,分化时间早且分化率高达到89.7%,生根时间短且根数多且壮,生根率达到44.7%。经4天炼苗后移栽到基质为草炭:蛭石:珍珠岩=2:1:1的穴盘内。25天观察发现红蓝7:9的红花山楂苗生长长势最好,整齐且高而壮;红蓝7:1+白光次之;红光下根长差达到最大值,说明长波光有利于植物伸长生长;蓝光下红花山楂的生长变化不大,说明短波光对红花山楂生长影响较小。综合分化、生根及移栽数据结果,得出红蓝比为7:9的光质为红花山楂生长的最适宜光质。

Using differentiated test tube seedlings of Crataegus cultivar as experimental material,the author investigated the effect of different light spectrum on differentiation,rooting,and growth after transplantation.The author hoped this research could shorten production cycle of plant tissue culture,enhance the quality of tissue seedlings,provide shortcut to fast propagation of other plant species,and offer theoretical basis and data support for the effects of light spectrum on plant growth.The results showed that the 7：9 ratio of red and blue light quality had the greatest impact on Crataegus differentiation and rooting in vitro,for the differentiation was earlier and the rate reached 89.7%,the rooting had more number and strong,the rate reached 44.7%.After 4 days transplanting to the matrix which mixed with the turf：vermiculite：perlite in 2：1：1.Observed after 25 days,the red and blue 7：9 seedlings grew best,tidiness and high strong;root length reached the maximum difference under the red light,that indicated the long waves promoted the plant extension length growth;their root fresh mass and root length decreased under blue light,which showed short wavelength light had less impacts on Crataegus growth.The author comprehensively considered the differentiation,rooting and transplanting,obtained the results for the 7：9 ratio of red and blue was the most appropriate light quality on growth of Crataegus.