温度、光照及继代周期等对金叶复叶槭组培苗生长的影响

Effect of Temperature,Subculture Cycle and Light on the Growth of Acer negundo 'Aureomarginatum' in Vitro

为了完善金叶复叶槭组培快繁技术、实现工厂化规模化生产,以其组培苗为试验材料,通过设置不同的温度、光照、继代周期等,研究培养条件对其生长的影响。结果表明,温度、继代周期、光照条件对苗高影响较大,差异均达到显著水平;同时发现继代周期显著影响叶片数量、光照时间对根生长系数影响差异达到极显著水平。筛选出在30℃、4200lx的光照强度下,16h/d光照时间、继代周期35天为‘金叶复叶槭最佳培养条件。

This paper aimed to study the tissue culture and rapid propagation technology of Acer negundo ＇Aureomarginatum＇and provided basis for the implementation of scale production.The effects of different temperature,subculture cycle,illumination and other culture conditions on the growth of the Acer negundo ＇Aureomarginatum＇in vitro were investigated.The results showed that the differences by temperature,subculture cycle,light on the plantlet height reached the significant level,it was significantly affected on leaf number by subculture cycle,and on root growth coefficient by illumination time.The optimal culture conditions for Acer negundo＇Aureomarginatum＇in vitro were at 30℃,4200 lx light intensity,16 h/d illumination time,and the subculture cycle was 35 days.