摘要
[目的]应用T-RFLP技术揭示小叶满江红内生细菌的多样性。[方法]将分离自实验室无菌培养的小叶满江红内生细菌进行16SrDNA-PCR扩增,产物用MspI、HhaI、HaeIII、HinfI 4种内切酶进行酶切,通过检测带荧光标记的片段,将所获得的数据用相关的软件工具Phylogenetic Assignment Tool(PAT)进行比对分析。[结果]通过比对获得10个科42个属的细菌菌种。[结论]T-RFLP技术配合生物信息学软件能较有效地揭示小叶满江红内生细菌的多样性。
[Objective] To explore the diversity of endophytic bacteria in Azolla microphylla by using T-RFLP.[Method] PCR products of DNA of endophytic bacteria in Azolla microphylla which were cultured by our laboratory in sterile conditions were digested with restriction endoenzyme MspI,HhaI,HaeIII,HinfI,respectively.The terminal fragment was analyzed with fluorescent through Phylogenetic Assignment Tool（PAT）.[Result] Bacteria belong to 10 Phylums 42 Classes were gained after analysis.[Conclusion] The genetic diversity of endophytic bacteria in Azolla microphylla could be figured out by using T-RFLP and bioinformatic software.
出处
《安徽农业科学》
CAS
2012年第29期14185-14187,14270,共4页
Journal of Anhui Agricultural Sciences
基金
福建省科技厅公益项目(2010R1021-8)
关键词
满江红
内生细菌
T-RFLP
Azolla microphyllla
Endophytic bacteria
T-RFLP
