铅对斑马鱼生殖系统毒性作用研究

Toxic Effect of Lead on Reproductive System in Zebra Fish

[目的]研究Pb对斑马鱼生殖系统的毒性作用。[方法]将成年斑马鱼(3月龄)随机分为4组,分别为对照(蒸馏水)组和低(0.4mg/L)、中(0.8 mg/L)、高(1.6 mg/L)剂量PbAc染毒组,每组20条,连续染毒7 d。染毒结束后,测定雌性斑马鱼产卵数和雄性斑马鱼产精子体积以及精子密度。[结果]与对照组相比,0.4和0.8 mg/L Pb染毒组斑马鱼产卵数较低,差异均有统计学意义(P<0.05);1.6mg/L Pb染毒组斑马鱼已不产卵。随着染毒剂量的增高,斑马鱼产卵数呈减少趋势。各Pb染毒组雄性斑马鱼精子密度与对照组比较,差异均无统计学意义。与对照组比较,0.4、0.8和1.6 mg/L Pb染毒组雄性斑马鱼精子体积均较低,差异有统计学意义(P<0.05)。置换交配试验结果也是类似。[结论]Pb染毒可对成年斑马鱼产生生殖毒性。

[Objective] To evaluate the toxic effect of lead on the reproductive system of mature zebra fish.[Method] Healthy adult zebra fish aged three months were randomly divided into one control group and three experimental groups.Twenty zebra fish in each group were treated with lead acetate solution at the dose of 0,0.4,0.8 and 1.6 mg/L for seven consecutive days.The zebra fish was sacrificed for the measurement of the sperm density,sperm volume and fecundity.[Result] The eggs counts produced by female zebra fish in 0.4 and 0.8 mg/L groups were both decreased significantly compared with the control group（P〈0.05）.1.6 mg/L groups did not produce any eggs at all.With the increase of lead concentration,a decreasing trend in sperm counts was observed.There were no statistical significances in sperm density between the experiment and the control groups;the sperm volume of 0.4,0.8 and 1.6 mg/L groups were all decreased significantly compared with the control group（P〈0.05）.With the lead administration dose increasing,a downtrend in the sperm volume was observed.The experiment of male and female replacement trials in seven days led to the same result.[Conclusion] Lead exposure can induce reproductive toxicity to mature zebra fish.