橙皮中类胡萝卜素的提取工艺研究

Extraction Technology of Carotenoids from the Orange Peel

[目的]确定溶剂法提取橙皮中类胡萝卜素的最佳工艺条件。[方法]以橙皮为原料,采用溶剂法提取其中的类胡萝卜素,通过单因素试验考察浸提溶剂种类、浸提料液比、浸提温度、浸提时间对类胡萝卜素得率的影响,再通过正交试验确定橙皮中类胡萝卜素提取的最优工艺。[结果]单因素试验得出,以丙酮为浸提溶剂,料液比为1∶15 g/ml,浸提温度为60℃,浸提时间为60 min时,类胡萝卜素得率最高。正交试验最终确定影响橙皮中类胡萝卜素提取的因素主次顺序依次为提取温度>料液比>提取时间,最优提取工艺条件为提取温度60℃,料液比为1∶20 g/ml,浸提时间为60 min。在该条件下,类胡萝卜素的得率达到97.3 mg/kg。[结论]该研究可为橙皮的综合利用提供借鉴和依据。

[Objective] To determine the best extraction technology of carotenoids from orange peel by solvent method.[Method] The carotenoids were firstly extracted from orange peel,and then the effects of solvent types,solid-liquid ratio,extraction temperature and extraction time on the yield of carotenoids were studied by single factor test,finally the best extraction conditions were determined by orthogonal experiment.[Result] As concluded from single factor test,the yield of carotenoids was the highest with the solvent of acetone under the solid-liquid ratio of 1：15（g/ml） and the extraction temperature of 60 ℃ for 60 min.As concluded from the orthogonal experiment,the influential degree of each factor was in the order of extraction temperature〉 solid-liquid ratio 〉 extraction time.The best extraction conditions were 60 ℃of extraction temperature,1∶20（g/ml） of solid-liquid ratio and 60 min of extraction time,under which the yield of carotenoids could reach up to 97.3 mg/kg.[Conclusion] The study provided references and basis for the comprehensive utilization of orange peel.