大孔树脂法纯化玉竹总皂苷的工艺研究

Purification of Total Saponins from Rhizoma Polygonati Odorati by Macroporous Resin Adsorption

目的:筛选出分离、纯化玉竹总皂苷的大孔树脂型号及工艺条件。方法:通过静态吸附与解吸实验确定大孔树脂的型号。在静态吸附与解吸单因素试验基础上,利用四因素四水平正交试验对LSA-33型大孔树脂纯化玉竹总皂苷的工艺进行研究,以总皂苷吸附率、解吸率为指标,确定最佳工艺。结果:LSA-33型树脂对玉竹总皂苷具有较好的纯化效果,其最佳工艺为:pH6～7的玉竹提取液,树脂吸附4h,解吸液为体积分数95%的乙醇,解吸液用量与树脂质量比37.5:1(mL/g),解吸时间为2h时,玉竹浸膏中总皂苷的含量由未纯化前的12.28%提高到41.75%。结论:LSA-33型大孔树脂可较好地纯化玉竹总皂苷,且操作简单、安全、成本低廉,有较高的应用价值。

Objectives： To find out the best type of macroporous resin and determine the optimal process conditions for separation and purification of total saponins from Rhizoma Polygonati Odorati. Methods： The best type of macroporous adsorption resin for total saponins was determined by static adsorption and desorption experiments. Based on one-factor-at-a- time experiments, a four-factor, three-level orthogonal array design was employed for the optimization of four process param- eters for improved adsorption and desorption rates. Results： LSA-33 rnacroporous resin was found to be the best resin among 6 types of macroporous resin tested. The best purification conditions for total saponins were determined as follows： crude extract from Rhizoma Polygonati Odorati （pH 6- 7） was adsorbed for 4 h and then desorbed with 95% ethanol at a solvent-to- solid ratio of 37.5：1 （mL/g） for 2 h. As a result, the content of total saponins in the crude extract was increased to 41.75% from 12.28%. Conclusion： LSA-33 macroporous resin is effective in purifying total saponins from Rhizoma Polygonati Odorati with the advantages of easy operation, high safety and low cost and thus will have high application value.