摘要
建立简单、快速测定酯交换植物油中高含量的植物甾醇的方法。对紫外比色法分析酯交换植物油中的植物甾醇含量进行研究,通过单因素与正交试验,确定最佳的测量条件为显色剂用量2mL、显色剂组成(铁矾储备液:浓硫酸)比例2:8、显色时间30min、显色温度20℃。测定结果与气相色谱法结果相比,相对误差平均偏高2.95%,校正后测定结果的相对误差为0.49%。本方法可用于酯交换植物油中甾醇的定量分析,操作简单、具有良好的准确性和实用性。
A simple and rapid colorimetric method was developed to determine phytosterol content in transestefified vegetable oil. The optimal experimental conditions for the determination of phytosterol content, as determined by one-factor-at-a-time and orthogonal array design methods, were as follows： the color developer used was composed of iron vitriol stock solution and concentrated sulphuric acid at a ratio of 2：8. the color development was allowed to proceed for 30 min at 20℃using 2 mL of the color developer. The results obtained by the colorimetric method presented a high relative error of 2.95% in comparison with those obtained by gas chromatography （GC）, and the average relative error was 0.49% after calibration. This method was easy, accurate, practical and suitable for the quantitative analysis of phytosterol in transesterified vegetable oil.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2012年第18期186-190,共5页
Food Science
基金
黑龙江省科技攻关项目(WB09B201-1)
关键词
酯交换植物油
紫外比色法
气相色谱法
植物甾醇
测定
transesterified vegetable oil
colorimetrics
gas chromatography （GC）
phytosterol
determination
