三种植物生长延缓剂对马铃薯试管苗生长和保存的影响

Effects of Three Plant Growth Retardantses on Tube-Plantlet Growth and Preservation of Potato

以MS为基本培养基,添加不同浓度的多效唑(PP333)、比久(B9)和甘露醇(GLC),比较其对脱毒马铃薯试管苗生长和保存的影响。结果表明:0.01～0.10mg/L PP333、5～20mg/L B9和1～10g/L GLC均对脱毒马铃薯试管苗有复壮和增殖双重效果,其中GLC的增值效果最为显著,最大增殖率达120%;0.1mg/L PP333和15～20mg/L B9利于移栽,植株生长旺盛、茎粗叶茂;0.1～1.0mg/L PP333、15～50mg/L B9和10～25g/L GLC适合于试管苗的保存。

To test effects of PP333, B9 and GLC on tube-plantlet growth and preservation of the virus-free potato,we added the three substances at different concentrations respectively to the base medium of MS. The results showed that PP333 （0.01 -0. 10mg/L）, B9 （5 -20mg/L） and GLC （1 -10g/L） could promote the rejuvenation and prolif- eration of the tube-plantlets of the virus-free potato, and GLC was most proliferation effective with the largest prolif- erate rate of 120% ;PP333 （0.1mg/L） or B9 （ 15 - 20mg/L） was advantageous to transplanting, the plantlet grew strong,the stem became thickset;PP333 （0.1 - 1.0mg/L）, B9 （ 15 - 50mg/L） or GLC （ 10 - 25g/L） was suitable for the preservation of the tube-plantlet.