摘要
筛选出 16株RB显色能力较强的菌株 ,测试了 16株菌的三种胞外木质素降解酶活性 ,结果显示 :侧耳菌侧 5、侧耳AX3、侧耳 810 1所产生漆酶活性最高 ;侧耳AX3、侧耳 810 1、金凤 2 5所产木质素过氧化物酶活性最高 ;侧耳 810 1、侧耳AX3、侧耳P4 6锰过氧化物酶活性最高 .综合结果表明 :木质素降解酶活性最高的菌株是侧耳 810 1和AX3.
Bavendamm reaction and Remazol brilliant blue (RB) fading method were used to select lignin degrading enzymes producers among forty five edible fungal strains. The results showed that 42 strains revealed RB fading ability and 16 strains displayed stronger of which three demonstrated the strongest. Then, all 16 strains were used to test their lignin degrading enzyme activities. The result showed that P.Ostreatus AX3,8101 and P5 strains producing laccase activity was the highest, P.Ostreatus AX3, P8101, Jinfeng 2 5 producing lignin peroxidase activity the highest and P.Ostreatus 8101, AX3, P46 producing Manganese peroxidase activity the highest. Finally, it was found that P.Ostreatus 8101 and AX3 strains produced the highest lignin-degrading enzymes activity.
出处
《四川大学学报（自然科学版）》
CAS
CSCD
北大核心
2000年第4期594-598,共5页
Journal of Sichuan University(Natural Science Edition)
关键词
木质素
侧耳菌
食用菌
降解酶
生物降解
laccase
lignin peroxidase
manganese peroxidase
pleurotus ostreatus
