不同基因缺失型酵母对全氟辛酸的灵敏性

Sensitivity of different gene deletion yeast to perfluorooctanoic acid

针对全氟化合物这类新型的有机污染物,在环境中具有持久性,生物累积性和毒性的问题,以全氟辛酸为模型化合物,以真核生物酵母以及4基因缺失型酵母突变体和5基因缺失型酵母突变体为模式生物,开发便捷、灵敏的毒理学评价方法,探究全氟辛酸对不同基因缺失型酵母的毒性效应,以期建立以酵母为平台的环境污染物快速筛选系统.研究结果表明,5基因缺失型酵母突变体对全氟辛酸较为敏感,可以作为建立环境污染物快速筛选系统的细胞模型.

Perfluorinated compounds （PFCs） are a class of emerging organic contaminants with persistent stability, strong bioaceumulation and high toxicity. To develop a convenient, sensitive toxicological evaluation method, perfluorooctanoic acid （PFOA）was used as a model compound and eukaryotie yeast and its 4 genes deletion yeast mutant and 5 genes deletion yeast mutant were used as model organisms. The toxic effects of PFOA on different gene deletion yeast were studied. The results show that 5 genes deletion yeast mutant is more sensitive to PFOA, which can be used as a cell model to establish rapid screening system of environmental pollutants.