姜油树脂中主要活性成分的GC/MS检测条件研究

A Conditional Study of Analyzing the Main Active Compositions in Ginger Oleoresin with Gas Chromatography-Mass Spectrometry

针对姜油树脂中烯类和酚类物质的特点,调整气相色谱-质谱联用方法的测试条件,获得了适于超临界CO2萃取的姜油树脂成分分析的GC/MS测试方法。在选定的检测条件下,可分析姜油树脂中主要的活性成分,检出物30多种。其中烯类主要成分α-姜黄烯(3.69%)、姜烯(17.11%)、α-法尼烯(2.28%)、β-没药烯(5.12%)和β-倍半水芹烯(7.77%)等倍半萜类化合物的分离效果好。姜辣素类的主要物质姜酚(7.70%)在检测过程中受热部分分解形成姜酮(30.36%)等热解产物,因此须同时计量分解和未分解部分。

In light of the characteristics of the olefins and pungent compounds in ginger oleoresin, the adjusted gas chromatography and mass spectrometry technique was employed to analyze the ginger o- leoresin products extracted by supercritical COz from ginger. More than 30 components were detected under the optimization conditions, α-curcumene （3. 69%）, zingiberene （ 17. 11%）, α-farnesene （2. 28 %）, β-bisabolene（5.12 %）, and β-sesquiphellandrene （7.77 %） the mainly sesquiterpene hydro- carbons active components of the olefins were precisely separated . The pungent compounds were mainly gingerol（7.70%） and zingerone（30.36%）produced by the thermal degradation of gingerols in the analysis. Consequently, the thermal degradation parts and undecomposed were taken into account simultaneously.