CD109对人宫颈鳞状细胞癌临床病理表现的影响

Influence of CD109 on the clinical pathological manifestations of human cervix squamous cell carcinoma

目的探讨CD109表达与人宫颈鳞状细胞癌临床病理表现之间的关系。方法选取天津医科大学附属肿瘤医院正常宫颈组织14例、宫颈上皮内瘤变3级(CIN3)组织14例、宫颈浸润性鳞状细胞癌及其淋巴结转移瘤组织12例。采用免疫组化法检测不同病理学分级宫颈组织中CD109和Ki-67的表达,并采用Western blotting进一步验证。采用Spearman等级相关分析CD109和Ki-67表达的相关性。结果 CD109的表达随病变进展逐渐增强,在宫颈浸润性鳞癌及其淋巴结转移瘤组织中的表达明显高于正常宫颈和CIN3组织中的表达(P<0.05)。Ki-67的表达随着宫颈病变的进展呈现增高趋势,各组间差异具有统计学意义(P<0.05)。CD109与Ki-67的表达阳性率呈正相关(r=0.953,P<0.01)。结论 CD109与宫颈鳞癌细胞增殖相关,且在宫颈鳞状细胞癌的发生、发展及浸润、转移过程中起重要作用。

Objective To explore the relationship between the expression of CD109 and the clinical pathological manifestations of human cervix squamous cell carcinoma. Methods Fourteen pieces of normal cervix tissue, 14 pieces of cervical intraepithelial neoplasia 3 （CIN3） tissue, 12 pieces of primary invasive cervix squamous carcinoma tissue and metastatic lymph-node tissue of cervix squamous cell carcinoma in Cancer Hospital of Tianjin Medical University were collected to conduct immunohistochemical staining for detecting the expression of CD 109. The results were further confirmed by Western blotting. The expression of Ki-67 in the aforesaid samples was detected by immunohistochemical staining and the relationship between CD109 and Ki-67 was analyzed. Results The CD109 expression gradually increased with tumor progression. The expression of CD109 were significantly higher in cervix squamous cell carcinoma tissue and metastatic lymph-node tissue than in normal cervix tissue and CIN3 tissue （P〈0.05）. The Ki-67 expression also increased with tumor progression. The differences in Ki-67 expression among the four groups were statistically significant （P〈0.05）. CD109 expression was positively correlated with the expression of Ki-67（r=0.gs3, P〈0.01）. Conclusions CD109 seems to correlate with the proliferation of cervix squamous carcinoma cell and may play an important role in the development, progression, invasion and metastasis of cervix squamous cell carcinoma.