不同程度邪毒证H22肝癌小鼠血液激素含量动态变化分析

Analysis of the Dynamic Changes of Blood Hormone Levels in H22 Liver Cancer Mice of Poisonous Pathogenic Factors Syndromes to Different Degrees

目的研究不同程度邪毒证肝癌小鼠血液激素含量动态变化的特点。方法 220只小鼠腋下接种H22肝癌腹水细胞,于接种后第9天筛选邪毒壅盛证与邪毒微弱证小鼠,另设正常组(30只)。分别在接种后第10、11天(第1、2时间窗口)处死小鼠,称量肿瘤和胸腺、脾脏湿重;ELISA法检测血浆促肾上腺皮质激素(ACTH)、皮质酮、醛固酮、睾酮、甲状腺激素T3与T4,以及TNF-α、IFN-γ含量,并对上述指标进行相关分析。结果同一时间窗口,邪毒壅盛证小鼠肿瘤质量明显大于邪毒微弱证(P≤0.05)。与正常组比较,2个时间窗口的邪毒壅盛证小鼠胸腺明显萎缩(P≤0.05),而脾脏明显肿大(P≤0.05),血浆ACTH含量显著升高(P≤0.05),而邪毒微弱证ACTH升高幅度小;血浆醛固酮含量呈现类似ACTH的趋势。在第1时间窗口,2个邪毒证组血浆皮质酮含量均显著升高(P≤0.05)。邪毒壅盛证小鼠血浆睾酮与T4均呈下降趋势。2个邪毒证组小鼠血浆TNF-α和IFN-γ水平均呈现升高趋势。相关分析发现,邪毒程度分别与气虚程度和T4呈负相关(r=-0.766,-0.738,P≤0.05),与ACTH呈正相关(r=0.635,P≤0.05);气虚程度与阳热程度呈正相关(r=0.632,P≤0.05);ACTH与T4呈负相关(r=-0.504,P≤0.05),睾酮与TNF-α呈正相关(r=0.619,P≤0.05)。结论在H22肝癌小鼠疾病进展过程中,邪毒证会自然发生且表现出程度上的差异,不同程度的邪毒证小鼠神经内分泌激素的紊乱与免疫功能的抑制随病程的进展呈动态变化趋势。

Objective To study the dynamic changes of blood hormone levels in H22 liver cancer mice of poisonous pathogenic factors syndromes （PPFS） to different degrees. Methods Two hundred and twenty mice were injected with H22 tumor cells from their armpits. On the ninth day after inoculation the mice of severe poison- ous pathogenic factors syndrome （SPPFS） and of mild poisonous pathogenic factors syndrome （MPPFS） were screened. Besides, another normal control group consisting of 30 mice was set up. The mice were killed on the tenth and eleventh day after inoculation （as the 1st and 2nd time window）. The weight of the tumor, the wet weight of the thymus and the spleen were weighed. The plasma adrenocorticotropic hormone （ACTH）, corticos- terone, aldosterone, thyroid hormone T3 and T4, testosterone, TNF-α, and IFN-y were detected by ELISA. All the aforesaid laboratory parameters were analyzed. Results The tumor weight was obviously larger in mice of the SPPFS group than in those of the MPPFS group at the same time window （ P≤0. 05）. Compared with the normal control group, the thymus was obviously atrophied （ P ≤ 0.05）, the spleen was significantly enlarged （ P ≤ 0.05）, the plasma ACTH significantly increased （P≤0.05） in the SPPFS group at the two time windows. But the increment of ACTH was less in the MPPFS group. The plasma corticosterone showed similar tendency as that of ACTH. At the 1st time window the plasma testosterone significantly increased in the two groups （ P≤0. 05）. The plasma testosterone and T4 showed a decreasing tendency in the SPPFS group. The plasma TNF-α and IFN-V levels showed an increasing trend in the two groups. Correlation study showed that the degree of PPFS was negatively correlated with qi deficiency （r= -0. 766, P≤0.05） and T4 （ r= -0. 738, P≤0.05）. The de- grees of PPFS was positively correlated with the plasma ACTH level （ r=0. 635, P≤0.05）. The degree of qi de- ficiency was positively correlated with yang heat syndrome （r=0. 632, P≤0. 05）. The plasma ACTH was nega- tively correlated with T4 （r= -0. 504, P〈~0. 05）. The plasma testosterone was positively correlated with TNF-c（ （r=0. 619, P≤0. 05）. Cenclmiem PPFS occurs naturally and shows difference to different degrees in the de- velopment of H22 liver cancer. The disorders of neuroendocrine hormones and the suppression of the immune function show dynamic changing trends.