依达拉奉对大鼠创伤性脑损伤作用的观察

The experimental study of edaravone injection in rats traumatic brain injury

目的:研究依达拉奉超早期应用对创伤性脑损伤继发性损伤(TBI)的保护作用。方法:大鼠随机分为假手术组、生理盐水组(TBI-NS,创伤后30 min、24 h静注生理盐水)、依达拉奉组(TBI-Ed,创伤后30 min、24 h静注依达拉奉)。伤后72 h处死部分大鼠,对伤侧皮层、海马及丘脑进行病理组织学检测。其余大鼠伤后15 d应用Morris水迷宫检测大鼠神经功能,同时监测不同时间活化部分凝血活酶时间(APTT)。结果:依达拉奉组皮层、海马及丘脑坏死神经元计数(F-J染色),水迷宫实验平均上台时间均较生理盐水组显著减少(P<0.05或P<0.01),但APTT与生理盐水组间无显著差异。结论:创伤性脑损伤后,超早期应用依达拉奉能显著减轻神经组织的病理损害,改善实验动物神经功能。

OBJECTIVE To investigate the effect of edaravone injection in the traumatic brain injury（TBI）.METHODS Rats were randomly divided into three groups： sham-operation group,saline group（TBI-NS,saline iv,30 min and 24 h post-injury）,edaravone group（TBI-Ed,edaravone injection iv,30 min and 24 h post-injury）.After 72 h survival,some rats were killed,brain samples（cerebral cortex,hippocampus and thalamus） were harvested for Fluoro-jade staining.Morris water maze was used to detect the neurological outcome after 15 d.APTT was measured in different time point after TBI.RESULTS Compared with saline group,necrosis neurons in cortex,hippocampus CA1 and thalamus area in edaravone group decreased significantly,the mean time on platform of Morris water maze decreased significantly（P0.05 or P0.01）.But APTT showed no changes between edaravone and saline group（P0.05）.CONCLUSION After brain injury,ultra-early use of edaravone injection could ameliorate pathological lesion and improve neurological outcome.