体外循环术中心肌再灌注损伤的观察

Observation of reperfusion injury during cardiopulmonary bypass in man

本文观察5例体外循环下心内直视手术患者术中血浆丙二醛(MDA)含量,共轭双键二烯(DCDB)含量、全血总超氧化物歧化酶(SOD)活力及血浆锰超氧化物歧化酶(Mn-SOD)活力,以观察外科心肌再灌注损伤的变比。结果显示:体外循环期间反映自由基损伤的血浆脂质过氧化物MDA及DCDB在再灌时段明显升高(P<0.05)而抗超氧阴离子酶SOD在再灌时段明显下降(P<0.05)。另外,作者首先检测并发现血浆Mn-SOD在再灌时段明显升高(P<0.05)。

To observe the changes of surgical reperfusion injury, plasma malonyl dialdehyde (MDA), dienes with conjugated double bond (DCDB), whole blood superxide dismutase (SOD) activity and plasma manganese-containing SOD (Mn-SOD) activity were determined during cardiopulmonary bypass (CPB) in 5 cases undergoing cardiosurgery. The results showed that reperfusion period on CPB was associated with a significant increase in the products of lipid peroxidation, MDA and DCDB (P<0.05 for each). However, it indicated that SOD of suppressed superoxide anion fell significantly (P<0.05). Of interest was the finding that plasema Mn-SOD activity rose significantly (P<0.05) during reperfusion period.