连翘组织培养研究Ⅰ 愈伤组织的诱导与培养条件的优化

Studies on the tissue culture of Forsythia suspensa (Thunb)Vahl (Oleaceae)Ⅰ Callus inducement and optimization of media

在MS培养基上附加不同种类和浓度配比的激素 ,2 0 0 0lx的光照条件下对连翘植物愈伤组织的继代培养和生长量进行了比较 .当 2 ,4 -D 3.0mg/L时 ,愈伤组织诱导率最高 ,达 1 0 0 %;当 2 ,4 -D 0 .5mg/L ,KT 0 .5mg/L和BA 2 .0mg/L时最适合继代和细胞生长 .结果还表明 :单一 2 ,4 -D的各种浓度对愈伤组织都有较高的诱导率 .不同激素组合对愈伤组织生长量和发生的状况有明显影响 .在固体培养基上 ,愈伤组织的生长周期为 2 8d .为连翘植物细胞悬浮培养建立了基础 ,为解决连翘植物的资源提供了新途径 .

Comparison on tissue culture of Forsythia suspensa(Thunb)Vahl.Results indicated inducement rate in medium MS added 3.0 mg/L 2,4-D were higher than others,and medium MS plus 0.5 mg/L 2,4-D,0.5 mg/L KT and 2.0 mg/L BA was the best benefit for subculture.Time course of cell growth in solid was twenty-eight days.The experiments were conducted under light(2 000 lx),at temperature in the range of 25 ℃.The result provided the possibility of cell suspension culture of F.suspensaand also paved a new alternative way for resource of F.suspensa.