大鼠主动脉钙化过程中骨保护素/NF-κB配体的受体变化及意义

OPG/RANKL in process of the rat arterial calcification

目的:研究大鼠主动脉钙化过程中骨保护素(OPG)/NF-κB配体的受体(RANKL)变化及意义。方法:60只SD大鼠随机分为钙化组、他汀组和对照组,每组再分早、晚期两个亚组。钙化组给予华法林灌胃,他汀组给予华法林和阿托伐他汀灌胃。早期亚组在第17天、晚期亚组在第34天取大鼠主动脉,用Von Kossa染色,以碱性磷酸酶(ALP)活性检测与骨钙素Western Blot检测来判定钙化情况,用实时定量PCR检测OPG和RANKL的表达情况。结果:对照组无论早晚期都有大量的OPG表达,但没有RANKL表达。钙化组和他汀组早期OPG表达上升,晚期下降,而RANKL表达在钙化过程中一直呈升高趋势;对照组、他汀组、钙化组随着钙化程度的加重,OPG/RANKL比值呈逐步下降趋势(均P<0.05);同一组中,晚期亚组随着钙化的加重,OPG/RANKL比值也较早期明显下降(均P<0.05)。结论:OPG/RANKL的比值与主动脉钙化程度呈负相关,且阿托伐他汀具有抑制钙化的作用。

Objective：To study the change and meaning of osteoprotegerin （OPG） receptor activator of NF-xB ligand （RANKL） during the process of the rat arterial calcification. Method：The 60 SD rats were divided randomly into control group, atorvastatin group and calcified group. Each group was also divided into the early sub-group and late sub-group. Next, the calcified group was taken the intragastric administration with Warfarin~ the atorvas- tatin group was taken the intragastric administration with both Warfarin and atorvastatin. The early and late sub- groups were detected at 17 days and 34 days, respectively. Von Kossa staining, ALP activity assay and osteocalcin assayed with Western Blot were used to detect the calcification level; Real Time PCR was used to detect the expression of OPG and RANKL. Result：There was great expression of OPG but no expression of RANKL in con- trol group neither early nor late sub-group. The expression of OPG was increased in the early sub-group and decreased in the late sub-group in atorvastatin group and calcified group. However, the expression of RANKL in these two groups was increased all the time during the calcification. Among the three groups, the ratio of OPG/ RANKL was decreased along with the increase of calcification level. And compared with the early sub-groups, the ratio of OPG/RANKL in the late sub-groups was also decreased along with the increase of calcification level in each group. Conelusion..The ratio of OPG/RANKL has a negative correlation with the level of the arterial calcifica- tion, and atorvastatin could significantly inhibit the arterial calcification.